产香酵母菌筛选、条件优化及发酵产物在电子烟烟液中的应用  

Aroma-producing yeasts:screening,optimization and application in e-liquids

作　　者：巩效伟 刀娅 赵伟 韩熠 朱东来 罗义勇[2] GONG Xiaowei;DAO Ya;ZHAO Wei;HAN Yi;ZHU Donglai;LUO Yiyong(Technology Center,China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650231,China;Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]云南中烟工业有限责任公司技术中心,昆明市650231 [2]昆明理工大学生命科学与技术学院,昆明市650500

出　　处：《烟草科技》2020年第2期62-71,共10页Tobacco Science & Technology

基　　金：云南中烟工业有限责任公司科技项目“特色功效型电子烟烟油的开发与应用”(2016XY02)。

摘　　要：为丰富电子烟烟液香气物质来源,开发具有良好风味的电子烟产品,筛选了食源性产香酵母菌,利用气相色谱质谱联用(GC/MS)和高效液相色谱(HPLC)分析致香成分,以关键致香成分为指标对产香条件进行正交试验优化,并将最优条件下获得的发酵产物进行电子烟加香效果评价。结果表明:(1)筛选获得1株能够以杨梅汁和葡萄汁为唯一营养物的酿酒酵母菌(Saccharomyces cerevisiae)KMLY1-2,该酵母菌发酵杨梅汁和葡萄汁后可产生较高水平的苯乙醇。(2)杨梅汁和葡萄汁发酵产苯乙醇的最优条件分别为接菌量5%和10%、温度30和25℃、转速200 r/min、pH 3和9;在此条件下,杨梅汁和葡萄汁的苯乙醇产量分别为47.49和60.99 mg/L,较未优化处理分别增加11.99和27.06 mg/L,较对照(未发酵杨梅汁或未发酵葡萄汁)分别增加47.32和60.99 mg/L。(3)最优条件下得到的发酵产物的嗅香得分最高;将发酵产物按1.6%(质量分数)比例添加至电子烟烟液基础溶剂中,制备的电子烟具有浓郁玫瑰花香,且产生的气溶胶香气谐调饱满,杂气轻,余味干净,抽吸品质好。酿酒酵母KMLY1-2可用于发酵杨梅汁和葡萄汁,发酵产物含较高浓度苯乙醇,是一种合适的电子烟香原料。In order to enrich the aroma of e-liquids and develop e-cigarettes with superior flavors,foodborne aroma-producing yeasts were screened,and the aroma components were analyzed by gas chromatography-mass spectrometry(GC/MS)and high performance liquid chromatography(HPLC).Using the key aroma compound as an indicator,the aroma-producing conditions were orthogonally optimized.Finally,the fermentation products obtained under the optimal conditions were evaluated for their sensory effects on e-cigarettes.The results showed that:1)A strain of Saccharomyces cerevisiae,KMLY1-2,was obtained by using waxberry juice and grape juice as nutrient separately,and KMLY1-2 could ferment waxberry juice and grape juice to produce higher levels of phenylethanol.2)The optimal conditions for producing phenylethanol in fermented waxberry juice and grape juice were 5%and 10%of inoculation amount,30 and 25°C fermentation temperature,rotation speed of 200 r/min,pH3 and 9,respectively.The phenylethanol contents in waxberry juice and grape juice fermented under the optimal conditions were 47.49 and 60.99 mg/L,which were 11.99 and 27.06 mg/L higher than those under the unoptimized conditions,47.32 and 60.99 mg/L higher than those in unfermented waxberry juice and grape juice,respectively.3)The olfactory aroma score of the fermentation products obtained under the optimal conditions was the highest.The e-cigarette samples prepared by adding the fermentation products into the base solvent of e-liquids at the rate of 1.6%(mass fraction)had rich rose flower scent,the generated aerosols featured harmonious and full aroma,low offensive odor,clean aftertaste and good smoking quality.Therefore,S.cerevisiae KMLY1-2 can be applied to the fermentation of waxberry juice or grape juice to enhance the concentration of phenylethanol,which could be evaluated for e-cigarette flavors.

关 键 词：酿酒酵母菌 苯乙醇 致香成分 电子烟烟液 香原料 

分 类 号：TQ656.5[化学工程—精细化工] Q939.9[生物学—微生物学]