茶多酚对急性镉(Ⅱ)暴露小鼠红细胞及肝脏损伤的拮抗作用  

Antagonistic Effect of Tea Polyphenols on Erythrocyte and Liver Injury in Acute Cadmium(Ⅱ)Exposure for Mice

作　　者：汪雪睿 黄保嘉 李想 王硕[2] 程代 Wang Xuerui;Huang Baojia;Li Xiang;Wang Shuo;Cheng Dai(State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology,Tianjin 300457;School of Medicine,Nankai University,Tianjin 300071)

机构地区：[1]天津科技大学省部共建食品营养与安全国家重点实验室,天津300457 [2]南开大学医学院,天津300071

出　　处：《中国食品学报》2020年第4期66-72,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金青年科学基金项目(31801453);中国博士后科学基金项目(2017M621059,2018T 110194)。

摘　　要：目的:旨在评价茶多酚(TP)对CdCl2诱导的小鼠急性红细胞损伤及肝脏损伤的保护作用。方法:以8周龄昆明小鼠为研究对象,首先通过单次腹腔注射CdCl2(30μmol/L Cd2+/kg)溶液建立急性镉暴露小鼠损伤模型,后续用TP(100 mg/kg)腹腔注射5 d进行干预。检测小鼠红细胞渗透脆性、红细胞形态、血液及肝脏中Cd(Ⅱ)水平。测定小鼠血清中转氨酶酶活,肝脏组织中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性及丙二醛(MDA),并进行肝脏病理切片的组织学检验。结果:与空白对照组相比,急性镉暴露后导致小鼠肝脏和血液中的镉元素含量、血清转氨酶活性和红细胞渗透脆性的显著增加,以及内源性抗氧化酶活性显著降低。然而,在急性镉暴露后用TP处理小鼠可减轻CdCl2的氧化损伤,维持红细胞细胞膜,显著提高抗氧化酶SOD、CAT活性,降低MDA水平,防止肝细胞组织形态学的改变。结论:TP不仅与Cd螯合,而且发挥抗氧化作用来减弱CdCl2对小鼠红细胞及肝脏组织的损伤。TP的摄入会降低Cd对血液系统和肝脏组织的损伤。Objective:This study was undertaken to determine the effectiveness of tea polyphenols(TP)in preventing cadmium chloride(CdCl2)induced hepatotoxicity and hematotoxicity in mice.Methods:Eight-week-old male Kunming mice were chosen.Control,Cd-treated(a single injection of 30μmol/L Cd2+/kg,i.p.),Cd+TP(after Cd treated,administered to mice daily for 5 days at 100 mg/kg,i.p.)and group of TP per se were used.Erythrocyte fragility,the influence on cellular biochemical components,lipid peroxidation level,morphological changes,the levels of cytosolic enzymes in serum,the extent of oxidative damage and levels of the key antioxidant enzymes in liver,and hepatic histopathology in mice were evaluated.Results:The levels of Cd(Ⅱ)in liver and blood,the activities of transaminases in serum and osmotic fragility were increased by comparison with the control,while the activities of superoxide dismutase and catalase decreased significantly in the Cd-treated group.However,treating mice with TP alleviate Cd-induced oxidative damaging effects,stabilize cell membrane,prevent the changes of hepatic histopathology.Conclusion:TP not only chelates with Cd,but also plays an antioxidant role to weaken the biotoxicity of CdCl2.TP supplementation may be favorable to avoid Cd-induced hematotoxicity and hepatotoxicity for humans.

关 键 词：镉 茶多酚 红细胞 肝脏 螯合 抗氧化 

分 类 号：R114[医药卫生—卫生毒理学]