枯草芽孢杆菌Z-3产木聚糖酶固态发酵工艺优化  

Optimization of solid-state fermentation technology of xylanase production by Bacillus subtilis Z-3

作　　者：罗璇 石玉 袁敏文 陈同 冯光志 LUO Xuan;SHI Yu;YUAN Minwen;CHEN Tong;FENG Guangzhi(School of Food and Biotechnology,Wuhan Institute of Design and Sciences,Wuhan 430205,China;Huangshi Food and Drug Inspection and Testing Center,Huangshi 435000,China)

机构地区：[1]武汉设计工程学院食品与生物科技学院,湖北武汉430205 [2]黄石市食品药品检验检测中心,湖北黄石435000

出　　处：《中国酿造》2020年第3期52-56,共5页China Brewing

基　　金：武汉设计工程学院科研项目(K201911);国家级大学生科技创新项目(201814035006)。

摘　　要：以木聚糖酶活力为响应值,通过单因素及响应面试验优化克氏原螯虾肠道来源枯草芽孢杆菌(Bacillus subtilis)Z-3产木聚糖酶的固态发酵工艺。结果表明,最优固态发酵工艺为培养时间3 d、培养温度37℃、初始pH值5.0、装料量30 g/250 mL,接种量2.5%,碳源为麸皮与玉米芯混合物,麸皮占比60%,氮源为氯化铵。在此最优发酵工艺条件下,木聚糖酶活力为3459.58 U/g湿基,较未优化前(2079.1 U/g湿基)提高66.40%。Using xylanase activity as the response value,the solid-state fermentation technology for xylanase production by Bacillus subtilis Z-3 isolated from the intestinal of Procambarus clarkii was optimized by single factor and response surface tests.The results showed that the optimal solid-state fermentation technology was culture time 3 d,temperature 37℃,initial pH value 5.0,loading volume 30 g/250 ml,inoculum 2.5%,bran and corn cob(bran 60%)as carbon source,and ammonium chloride as nitrogen source.Under the optimal fermentation conditions,the xylanase activity was 3459.58 U/g wet basis,which was 66.40%higher than that before optimization(2079.1 U/g wet basis).

关 键 词：木聚糖酶 枯草芽孢杆菌 固态发酵 响应面法 

分 类 号：Q939.99[生物学—微生物学]