响应面优化‘不知火’柑橘果肉渣中结合酚的碱法提取工艺  

Optimization of alkaline extraction process for bound phenolics from‘dekopon’citrus pulp residue

作　　者：钟宜辰 彭颖[1] 张珮珮[1] 潘思轶[1] ZHONG Yichen;PENG Ying;ZHANG Peipei;PAN Siyi(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学食品科学技术学院,武汉430070

出　　处：《华中农业大学学报》2020年第2期133-140,共8页Journal of Huazhong Agricultural University

基　　金：湖北省技术创新专项(109-409116012)。

摘　　要：为提高‘不知火’柑橘果肉渣中酚类物质的利用价值,采用已去除游离酚的‘不知火’柑橘果肉渣作为原料,首先比较了不同溶剂提取对结合酚含量的影响,其次通过单因素试验和响应面分析法优化结合酚的碱法提取工艺,最后利用HPLC法分别检测结合酚与游离酚中单体酚的种类和含量。结果发现:NaOH溶液作为提取溶剂对结合酚的提取效果相对最好;液料比22∶1(mL/g)、NaOH浓度4.1 mol/L、碱解时间3.2 h时,结合酚含量达356.51 mg/100 g;游离酚提取液中检测出10种单体酚,其中橙皮苷的含量最高(284.44μg/g),阿魏酸则未被检测到;结合酚提取液中检测出8种单体酚,柚皮苷二氢查尔酮的含量最高(297.29μg/g),阿魏酸含量高达128.07μg/g,而绿原酸、原儿茶酸未被检测到。综上表明,结合酚的酚酸组成丰富,酚酸含量明显高于游离酚,尤其是阿魏酸含量。In order to improve the utilization value of bound phenolics from‘dekopon’citrus pulp residue,the‘dekopon’citrus pulp residue without free phenolics was used as raw materials.The effects of different solvent extractions on the content of bound phenolics were compared.Subsequently,the single-factor test and response surface analysis method were used to optimize the alkaline extraction process.And the type and content of monomeric phenol in bound phenolics and free phenolics were detected by HPLC method.The results showed that NaOH solution was the best extraction solvent for the extraction of bound phenolics.At a liquid-to-material ratio of 22 mL/g,NaOH concentration of 4.1 mol/L,and alkaline hydrolysis time of 3.2 h,the content of bound phenol reached 356.51 mg/100 g.Ten monomeric phenols were detected in the free phenolics extract,among which hesperidin was the highest(284.44μg/g),and ferulic acid was undetectable.Eight monomeric phenols were detected in the combined phenol extract.The content of naringin dihydrochalcone was the highest(297.29μg/g)and the content of ferulic acid reached as high as 128.07μg/g.Chlorogenic acid,protocatechuic acid were undetectable.In summary,the phenolic acid composition of bound phenolics is rich,and the content of phenolic acid is significantly higher than that of free phenolics,especially the ferulic acid content.

关 键 词：柑橘 果肉渣 结合酚 游离酚 响应面法 碱法提取 

分 类 号：TS255.2[轻工技术与工程—农产品加工及贮藏工程]