Linggui Zhugan Decoction(苓桂术甘汤)Inhibits Ventricular Remodeling after Acute Myocardial Infarction in Mice by Suppressing TGF-β1 Smad Signaling Pathway  

作　　者：WANG Liang SHI Hui HUANG Jin-ling XU Shan LIU Pei-pei 

机构地区：[1]College of Integrated Chinese and Western Medicine,Anhui University of Chinese Medicine,Hefei(230012),China [2]Anhui Province Key Laboratory of Chinese Medicinal Formula,Hefei(230012),China [3]Graduate School,Anhui University of Chinese Medicine,Hefei(230012),China

出　　处：《Chinese Journal of Integrative Medicine》2020年第5期345-352,共8页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundatio of China(No.30973707,81373533,81202631);the Natural Science Foundation of Anhui Province(NO.1508085QH192,1608085QH222)。

摘　　要：Objective:To investigate the inhibitory effect of Linggui Zhugan Decoction(LZD,苓桂术甘汤)on the ventricular remodeling(VR)after acute myocardial infarction(AMI)and related mRNA and proteins expression in transforming growth factor-beta 1(TGF-β1)/Smad signaling pathway,and explain its putative mechanism.Methods:A VR model was generated by ligation of coronary artery in mice.Two weeks after surgery,60 mice were randomly divided into the model group,the sham-operation group(distilled water),the positive control group(2.4 mg/kg simvastatin),and the low-,medium-and high-dose LZD groups(2.1,4.2,8.4 g crude drug/kg,respectively)by a random number table,10 mice in each group.Mice in each group was treated for 4 weeks.Changes of hemodynamics indices and cardiac weight index were detected by the PowerLab data acquisition and analysis recording instrument.Morphology changes of myocardial tissue were observed by hematoxylin-eosin and Masson staining.The expressions of TGF-β1,Smad2,Smad3,p-Smad2 and p-Smad3 in myocardial tissue were detected by Western blotting.The mRNA expressions of TGF-β1,Smad2 and Smad3 were detected by reverse transcription-quantitative polymerase chain reaction(RT-qPCR).The expressions of matrix metalloprotein 2(MMP2),MMP9,collagenⅠand collagen Ⅲ were observed by immunohistochemical methods.Results:VR mice showed significant dysfunction in hemodynamic indices and cardiac structure and function.Compared with the shamoperation group,myocardial tissue damage,interstitial fibrosis occurred in the model mice,left ventricular systolic pressure(LVSP),left ventricular pressure maximum contraction rate(+dp/dtmax)and left ventricular pressure maximum relaxation rate(-dp/dtmax)decreased significantly(all P<0.01),while left ventricular end-diastolic pressure(LVEDP),cardiac weight index and left ventricular weight index elevated significantly,meanwhile TGF-β1,p-Smad2,p-Smad3,Smad2,Smad3,MMP2,MMP9,collagen Ⅰ,collagen Ⅲ protein expressions in myocardial tissue and TGF-β1 Smad2 and Smad3 mRNA express

关 键 词：Linggui Zhugan DECOCTION acute myocardial INFARCTION VENTRICULAR REMODELING transtorming growth FACTOR-BETA 1/Smad signaling PATHWAY Chinese medicine 

分 类 号：R285.5[医药卫生—中药学]