低温等离子体对金黄色葡萄球菌肠毒素B的灭活作用  

Inactivation of Staphylococcal Enterotoxin B by Low Temperature Plasma

作　　者：潘迪 张大革[1] 孙运金[1] 马挺军[1] PAN Di;ZHANG Da-ge;SUN Yun-jin;MA Ting-jun(College of Food Science and Technology,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]北京农学院食品科学与工程学院,北京102206

出　　处：《食品工业科技》2020年第10期192-195,202,共5页Science and Technology of Food Industry

摘　　要：采用金黄色葡萄球菌肠毒素B(SEB)休克小鼠模型评价低温等离子体对SEB的灭活效果。实验设定为空白对照组、阳性组和3个处理组(等离子体降解处理SEB 10、20、30 min组),小鼠注射SEB后,观察120 h。记录各组小鼠存活时间和体重,并测定小鼠肝脏中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)的活性。结果表明:SEB的含量随等离子体处理时间的延长而降低,在处理30 min时SEB的降解率最大为96%。与阳性组相比,处理SEB 10、20、30 min组小鼠平均生存时间均显著延长(P<0.05),分别为阳性组的2.50、3.33、5.00倍;处理SEB 30 min组小鼠平均体重显著高于阳性组(P<0.05);处理SEB 30 min组小鼠肝脏中CAT、SOD、GSH-Px的活性与阳性组相比显著提高(P<0.05),分别提高了69.33%、74.93%、47.92%。综上,低温等离子体处理可以降低SEB的含量,从而提高小鼠体内抗氧化酶活性,增强机体免疫力,延长存活时间,且其存活时间具有处理时间依赖性。Staphylococcal enterotoxin B(SEB)shock mouse model was used to evaluate the inactivation effect of low temperature plasma on SEB.The experiment was set as blank control group,positive group and three treatment groups(plasma degradation treatment of SEB for 10,20 and 30 min groups),observing for 120 h after injecting SEB,the survival time and the weight of every mouse was recorded.And the activities of catalase(CAT),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in the mice liver were measured.The results showed that the contents of SEB were decreased with the prolonging of plasma treatment time,and the maximum degradation rates of SEB were 96%when SEB was treated for 30 min.Compared with the positive group,the mean survival time of mice in treatment SEB for 10,20 and 30 min groups was significantly extended(P<0.05),which was 2.50,3.33 and 5.00 times of the positive group,respectively.The average body weight of mice in treatment SEB for 30 min group was significantly increased than that in positive group(P<0.05).And the activity of CAT,SOD and GSH-Px in the liver of mice in treatment SEB for 30 min group was significantly increased(P<0.05),which was 69.33%,74.93%and 47.92%higher than that in the positive group,respectively.The conclusion was that low temperature plasma treatment could reduce SEB contents,so as to improve the activity of antioxidant enzyme in mice,enhance body immunity and prolong survival time which had time-dependent.

关 键 词：低温等离子体 金黄色葡萄球菌肠毒素B 介质阻挡放电 灭活效果 抗氧化活性 

分 类 号：TS207.7[轻工技术与工程—食品科学]