苦瓜皂甙的提取及其对胰脂肪酶抑制活性的研究  

Extraction of Momordica Saponins and Its Inhibitive Ability on Pancreatic Lipase

作　　者：周巧杰 吴子健[1] 胡佩卿 侯惠静 ZHOU Qiao-jie;WU Zi-jian;HU Pei-qing;HOU Hui-jing(College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin Key Laboratory of Food Biotechnology,Tianjin 300134,China;Guangdong Biolight Meditech Co.,Ltd.,Tianjin 300134,China;School of Bioengineering and Food Engineering,Tianjin Tianshi College,Tianjin 301700,China)

机构地区：[1]天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津300134 [2]广东宝莱特医用科技股份有限公司,天津300134 [3]天津天狮学院食品工程学院,天津301700

出　　处：《食品研究与开发》2020年第10期69-74,共6页Food Research and Development

基　　金：国家自然科学基金(面上项目)(31772019)。

摘　　要：苦瓜皂甙可有效抑制胰脂肪酶的活力,是一种潜在的减肥辅助食物补充剂。通过响应面法对苦瓜皂甙的最佳提取工艺进行优化,并测定其对胰脂肪酶的抑制率。研究结果表明:苦瓜皂甙的最佳提取工艺为乙醇提取液的浓度为50%、料液比1∶13.5(g/mL)、提取温度38℃、浸提时间77 min、浸提次数1次,此条件下苦瓜皂甙的提取产率为1.419 1%,其对胰脂肪酶抑制的IC50值为0.384 7 mg/mL。Momordica saponins can effectively inhibit pancreatic lipase activity and is a potential weight loss supplementary food. Response surface methodology was used to optimize ethanol extraction of saponin from bitter Momordica charantia,and later the inhibition of pancreatic lipase by saponin was determined. Results showed that the optimal extraction conditions were 38 ℃ for 77 min in one operation,with an Momordica charantia powder-to-ethanol solvent ratio being 1 ∶ 13.5(g/mL)and the ethanol concentration of extraction solvent being 50 %. Under these conditions,the extraction rate of Momordica saponins was 1.419 1 % and its IC50 value for pancreatic lipase inhibition was 0.384 7 mg/mL.

关 键 词：苦瓜 响应面法 胰脂肪酶 苦瓜皂甙 抑制率 

分 类 号：TS201.2[轻工技术与工程—食品科学]