苦丁皂苷L、苦丁皂苷N与牛血清白蛋白的相互作用研究  

Study on the Interaction between Kudinoside L,Kudinoside N and Bovine Serum Albumin

作　　者：胡夏恬 倪帅帅 焦安妮 于敏[2] 关玥[2,3] 张凤清[1] 焦连庆[2] HU Xia-tian;NI Shuai-shuai;JIAO An-ni;YU Min;GUAN Yue;ZHANG Feng-qing;JIAO Lian-qing(College of Chemistry and Life Sciences,Changchun University of Technology,Changchun 130012,China;Jinlin Provincial Academy of Traditional Chinese Medicine,Changchun 130012,China;College of Chinese Herbal Medicine,Jinlin Agricultural University,Changchun 130118,China;College of Pharmacy,Jinlin University,Changchun 130021,China)

机构地区：[1]长春工业大学化学与生命科学学院,吉林长春130012 [2]吉林省中医药科学院,吉林长春130012 [3]吉林农业大学中药材学院,吉林长春130118 [4]吉林大学药学院,吉林长春130021

出　　处：《食品工业科技》2020年第9期55-60,共6页Science and Technology of Food Industry

基　　金：吉林省科技发展计划(20150311037YY);吉林省卫生计生科研计划项目(2014z206);吉林省中医药科技项目(2014-ZD28)。

摘　　要：为研究不同温度下苦丁皂苷L(kudinoside L)、苦丁皂苷N(kudinoside N)与牛血清白蛋白(BSA)间结合和转运的机制及BSA二级结构变化情况。采用荧光光谱法研究kudinoside L、kudinoside N与BSA间荧光猝灭机制;采用圆二色谱法研究BSA二级结构的变化情况。结果表明kudinoside L、kudinoside N均能有效猝灭BSA内源荧光,猝灭类型为动态猝灭,分子间作用力主要为疏水作用,两个化合物与BSA各形成1个结合位点。此外,kudinoside L与BSA结合后,BSA中的α-螺旋和β-折叠含量增加;kudinoside N与BSA结合后,BSA中α-螺旋和无规卷曲含量减少,表明上述两种皂苷与BSA结合后,BSA内部结构环境均发生了改变。In order to study the mechanism of binding and transport between kudinoside L,kudinoside N and bovine serum albumin(BSA)and the secondary structure of BSA at different temperatures.The fluorescence quenching mechanism between kudinoside L,kudinoside N and BSA was studied by fluorescence spectroscopy.The change of secondary structure of BSA was studied by circular dichroism.The results showed that both kudinoside L and kudinoside N could effectively quench the endogenous fluorescence of BSA.The quenching type was dynamic quenching,the intermolecular force was mainly hydrophobic,and the two compounds formed a binding site with BSA.In addition,after kudinoside L combined with BSA,theα-helix andβ-sheet content in BSA increased;after kudinoside N combined with BSA,theα-helix and random coil content in BSA decreased.It was proved that the internal structure and environment of BSA changed after the combination of the above two saponins with BSA.

关 键 词：苦丁皂苷L 苦丁皂苷N 牛血清白蛋白 荧光光谱法 圆二色谱法 

分 类 号：TS201.3[轻工技术与工程—食品科学]