鼠李糖乳杆菌对大鼠黄曲霉毒素B1促排效果的评价  

Evaluation of the Effect of Lactobacillus rhamnosus on Promoting the Excretion of Aflatoxin B1 in Rats

作　　者：张居典 刘亚文 王喜泉 徐珒昭 徐境含 武明月[1] 许晓曦[1] ZHANG Judian;LIU Yawen;WANG Xiquan;XU Jinzhao;XU Jinghan;WU Mingyue;XU Xiaoxi(School of Food Science,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Green Food Science Research Institute,Harbin 150028,China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]黑龙江省绿色食品科学研究院,黑龙江哈尔滨150028

出　　处：《食品科学》2020年第9期119-125,共7页Food Science

摘　　要：通过对连续低剂量摄入黄曲霉毒素B1(aflatoxin B1,AFB1)的大鼠每日补充不同处理方式及不同菌体浓度的鼠李糖乳杆菌(Lactobacillus rhamnosu),并根据大鼠血清生化及免疫等指标,确定鼠李糖乳杆菌降低大鼠体内AFB1的能力及效果。大鼠(空白组除外)给予含50μg/kg AFB1的饲料,并分别灌胃0.5 mL不同处理方式及不同菌体浓度的鼠李糖乳杆菌菌液。以大鼠体质量、血清细胞免疫因子、免疫球蛋白(immunoglobulin,Ig)等指标进行对比,探讨不同处理方式、不同菌体浓度的鼠李糖乳杆菌对大鼠生长状况及免疫调节作用的影响。结果表明:高剂量鼠李糖乳杆菌活菌对大鼠体内AFB1的吸附能力随菌体浓度变化差异较明显,高剂量活菌维持大鼠健康状况、肝功能和免疫功能稳定的效果最佳;灭活鼠李糖乳杆菌对大鼠体内AFB1有一定的吸附能力,在维持天冬氨酸转氨酶活力以及IgM、IgG质量浓度等指标稳定方面效果显著,但对细胞免疫因子效果不明显;低剂量鼠李糖乳杆菌发酵液对大鼠体内AFB1的吸附效果较差,仅高剂量发酵液有一定的吸附作用。Rats with continuous low-dose intake of aflatoxin B1(AFB1)were supplemented daily with live and inactivated suspensions of Lactobacillus rhamnosus and its cell-free culture supernatant at different concentrations,separately.To evaluate whether and how effectively Lactobacillus rhamnosus could reduce AFB1 in rats,serum biochemical and immune indicators were measured.All animals except those in the control group were given AFB1 at a dose of 50μg/kg and administered intragastrically with Lactobacillus rhamnosus at a volume of 0.5 mL.The effects of Lactobacillus rhamnosus and its cell-free culture supernatant at different concentrations on the growth and immune function of rats were evaluated by determining body mass,serum cytokine levels and immunoglobulin(Ig)levels.The results showed that live Lactobacillus rhamnosus had a good adsorption capacity for AFB1 in rats and its effects varied significantly with its concentration.Live Lactobacillus rhamnosus at high dose had the best effect on maintaining the health status,liver function and immune function of rats.Although inactivated Lactobacillus rhamnosus also had certain adsorption capacity for AFB1 and significantly maintained aspartate aminotransferase(AST)activity,as well as IgM and IgG in rats,it was ineffective for cytokine stabilization.In contrast,the low concentration cell-free culture supernatant of Lactobacillus rhamnosus had a poor adsorption effect toward AFB1 in rats,which was effective only at high concentration.

关 键 词：鼠李糖乳杆菌 黄曲霉毒素B1 吸附 免疫系统 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]