生物法制造丁二酸研究进展  

Recent progress in microbial production of succinic acid

作　　者：张耀 邱晓曼 陈程鹏 于卓然 洪厚胜[1,2,3] ZHANG Yao;QIU Xiaoman;CHEN Chengpeng;YU Zhuoran;HONG Housheng(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211816,Jiangsu,China;National Engineering Technique Research Center for Biotechnology,Nanjing Tech University,Nanjing 211816,Jiangsu,China;Nanjing Highke Bioengineering Equipment Co.,Ltd.,Nanjing 210009,Jiangsu,China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211816 [2]南京工业大学国家生化工程技术研究中心,江苏南京211816 [3]南京汇科生物工程设备有限公司,江苏南京210009

出　　处：《化工学报》2020年第5期1964-1975,共12页CIESC Journal

基　　金：国家高技术研究发展计划项目(2012AA021201)。

摘　　要：丁二酸因其C4分子结构在化工领域的潜在价值,被认为是一种具有广阔应用前景的生物基平台化合物。结合生物基丁二酸的产业现状,综合分析了丁二酸生产菌种及菌株改造、生物过程优化和丁二酸分离纯化这三个方面的研究现状。重点介绍以大肠杆菌、产琥珀酸放线杆菌、解脂耶氏酵母为代表的主要生产菌株及其改造策略;低值生物质利用及控制发酵过程中CO2供给和pH调节等生物过程优化策略;包括钙盐法、电渗析法、直接分离法等方法在内的丁二酸分离工艺。同时指出未来的研究重点将综合考虑经济性与能耗问题,将菌株与发酵和分离全过程整合,提高丁二酸产量,降低发酵及分离成本,进一步拓展生物基丁二酸市场应用领域。Succinic acid is considered to be a promising bio-based platform compound due to its potential value in the chemical industry for its C4 molecular structure. This article comprehensively analyzed the domestic and foreign scholars biological process optimization and separation process of succinic acid. It emphatically introduced the main production strains and their modification strategies, such as Escherichia coli, Actinobacillus succinogenes and Yarrowia lipolytica. It included the biological process optimization programs such as low biomass utilization, two stages and low pH fermentation strategy and CO2 supply and pH regulation during fermentation. And it also included the separation process of succinic acid such as calcium salt method, electrodialysis method and direct separation method. At the same time, it pointed out that the emphasis of the future research will be to comprehensively consider the economic and energy consumption issues, integrate the whole process of strain fermentation and separation, increase the yield of succinic acid, reduce the cost of fermentation and separation and then to further expand the market of bio-based succinic acid.

关 键 词：丁二酸 发酵 生产菌株 代谢工程 生物过程 下游过程 

分 类 号：Q599[生物学—生物化学] TQ921[轻工技术与工程—发酵工程]