大米蛋白水解条件的响应面法优化  

Optimization of Enzymatic Hydrolysis for Rice Protein via Response Surface Methodology

作　　者：黄艳燕[1] 王升 冯涛 唐智慧 莫君明 HUANG Yanyan;WANG Sheng;FENG Tao;TANG Zhihui;MO Junming(National Engineering Research Centre for Non food Biorefinery,State Key Laboratory of Non food Biomass and Enzyme Technology,Guangxi Academy of Sciences,Nanning,Guangxi,530007,China;Nanning Pangbo Biological Engineering Co.,Ltd.,Nanning,Guangxi,530004,China;Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,School of Chemistry and Chemical Engineering,Guangxi University for Nationalities,530006,China;Nanning Chemmatic Biotec Co.,Ltd.,Nanning,Guangxi,530006,China)

机构地区：[1]广西科学院,非粮生物质酶解国家重点实验室,国家非粮生物质能源工程技术研究中心,广西南宁530007 [2]南宁庞博生物工程有限公司,广西南宁530004 [3]广西民族大学化学化工学院,广西林产化学与工程重点实验室,广西南宁530006 [4]南宁穗肽生物技术有限公司,广西南宁530006

出　　处：《广西科学》2020年第2期175-181,194,共8页Guangxi Sciences

基　　金：国家自然科学青年基金项目(21702033);广西自然科学基金回国基金项目(2016GXNSFCA380018);广西高校引进海外高层次人才“百人计划”项目(桂教人〔2016〕59号);广西民族大学引进人才科研启动项目(2016MDQD003)资助。

摘　　要：运用响应面法优化大米蛋白酶法水解条件,提高大米蛋白水解度和提取率。本研究首先应用单因素实验法分析酶添加量、温度、pH值以及酶解时间对大米蛋白水解的影响;然后在单因素实验基础上,进一步采用Box Behnken法进行实验设计,考察上述4个因素对大米蛋白水解度和蛋白质提取率的影响。研究结果表明最佳酶解条件为温度62℃,酶添加量2.5%,pH值8.2,酶解时间10.5 h,此时大米蛋白的水解度可达到41.5%,蛋白质提取率可达93.1%。研究成果可为酶解制备可溶性大米蛋白肽的工业化应用提供参考。In order to improve the degree of rice protein hydrolysis and extraction rate,response surface method was used to optimize the hydrolysis process of rice proteins.In this study,first of all,the single factor experiment method was used to analyze the effect of enzyme addition amount,temperature,pH value and enzymatic hydrolysis time on rice protein hydrolysis.Furthermore,on the basis of single factor experiment,Box Behnken method was used to design the experiment to investigate the influence of the above four factors on the degree of rice protein hydrolysis and protein extraction rate.The results showed that the optimum conditions of enzymatic hydrolysis were temperature 62℃,enzyme addition 2.5%,pH 8.2,and hydrolysis time 10.5 h.Under this condition,the degree of hydrolysis of rice protein could reach 41.5%,and the rate of protein extraction could reach 93.1%.The research results can provide references for the industrial application of soluble rice protein peptide prepared by enzymatic hydrolysis.

关 键 词：响应面法 大米蛋白 酶解 水解度 蛋白质提取率 

分 类 号：S565.1[农业科学—作物学]