母乳婴儿源乳杆菌的筛选及其免疫调节能力研究  

Screening of Potential Probiotic Lactobacillus and Its Research on Immunomodulatory Activity in Vitro from Breast-fed Infant Faeces

作　　者：张娜 张波[1,3] 赵丽娜 王妙姝[4] 康红艳 田洪涛 罗云波 Zhang Na;Zhang Bo;Zhao Lina;Wang Miaoshu;Kang Hongyan;Tian Hongtao;Luo Yunbo(College of Food Science and Technology,Agricultural University of Hebei,Baoding 071000;Hebei 2College of Biochemistry&Environmental Engineering,Baoding University,Baoding 071000;Hebei 3National Engineering Research Center for Agriculture in Northern Mountainous Areas,Baoding 071000;Hebei 4New Hope Hebei Tianxiang Dairy Co.,Ltd.,Baoding 071000;Hebei 5College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083)

机构地区：[1]河北农业大学食品科技学院,河北保定071000 [2]保定学院生物化工与环境工程学院,河北保定071000 [3]国家北方山区农业工程技术研究中心,河北保定071000 [4]河北新希望天香乳业有限公司,河北保定071000 [5]中国农业大学食品科技与营养工程学院,北京100083

出　　处：《中国食品学报》2020年第5期29-35,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：河北省自然科学基金项目(C2017204094)。

摘　　要：以分离自母乳婴儿源的乳杆菌——鼠李糖乳杆菌BF-1、植物乳杆菌BF-15、唾液乳杆菌BF-29、干酪乳杆菌BF-55为对象,研究这些菌株对体外模拟人工胃、肠液及胆盐的耐受性,对Caco-2细胞的黏附能力、安全性和对小鼠脾淋巴细胞增殖活性的影响,探讨菌株的免疫调节活性。试验结果表明:植物乳杆菌BF-15对人工消化液和胆盐有较强的耐受性,对Caco-2细胞黏附能力[(7.10±0.30)CFU/cell]显著高于阳性对照菌株LGG[(3.90±0.30)CFU/cell](P<0.05);BF-15除对氨基糖苷类、糖肽类抗生素的固有耐药性外,对苯唑西林、头孢噻吩也有耐药性,无抗性质粒;BF-15和LGG的活性和热致死菌在一定的菌浓范围(1×10^6~10^7CFU/mL)均促进体外小鼠淋巴细胞增殖,表现出剂量依赖关系,同时活性菌株作用效果明显优于热致死菌株。在相同菌浓条件下,两株菌体外免疫调节能力没有显著性差异(P>0.05)。母乳婴儿源乳杆菌——植物乳杆菌BF-15具有较强的抗逆性,较高的黏附性和一定的免疫调节能力。The probiotic potential of four Lactobacillus from breast-fed infants faeces:Lactobacillus rhamnosus BF-1,Lactobacillus plantarum BF-15,Lactobacillus salivarius BF-29 and Lactobacillus paracasei BF-55 was evaluated by a series of in vitro assays:resistance to simulated gastrointestinal juice and bile,adhesion to Caco-2 cells,the safety and the effect on proliferative capacity of the spleen lymphocytes in th-e mice.Results showed that it was indicated that:Lactobacillus plantarum BF-15 has stronger tolerance toartificial digestive juice and bile salts.The adhesion to Caco-2 cells[(7.10±0.30)CFU/cell]of BF-15 was significantly higher than the positive control strain LGG[(3.90±0.30)CFU/cell](P<0.05).In addition,Lactobacillus plantarum BF-15 was resistant not only to aminoglycoside inherently,but also to oxacillin and cephalothin without plasmid.In a certain concentration range(1×10^6-10^7 CFU/mL),viable or heat-killed Lactobacillus both could promote lymphocyte proliferation of the mice in vitro and showed a dose-dependent relationship.Under the same bacterium concentration,there was no significant difference in immunoregulation ability between the two strains(P>0.05).Lactobacillus plantarum BF-15 presents favorable strain-specific properties,which could provide probiotic resources for developping new infants and young children formula milk powderin and new probiotic products in the future.

关 键 词：母乳婴儿源 植物乳杆菌 耐受性 粘附能力 淋巴细胞增殖 

分 类 号：TS201.3[轻工技术与工程—食品科学]