分子量对海参岩藻聚糖硫酸酯在体内吸收的影响  

Effect of molecular weight on absorption and metabolism of sea cucumber fucoidan

作　　者：王亚囡 仇文峰 杨毅 张鸿伟 石德玲 赵雪[1] WANG Ya-nan;QIU Wen-feng;YANG Yi;ZHANG Hong-wei;SHI De-ling;ZHAO Xue(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Qingdao Customs Technology Cente,Qingdao 266001,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]青岛海关技术中心,山东青岛266001

出　　处：《中国海洋药物》2020年第2期42-49,共8页Chinese Journal of Marine Drugs

基　　金：国家重点研发计划(2018YFC0311201);山东省重点研发计划项目(2018YYSP006,2016YYSP017);浙江省自然科学基金(LY19C200001)资助。

摘　　要：目的利用高温高压降解法制备2种不同分子量的岩藻聚糖硫酸酯,探究口服不同分子量的海参岩藻聚糖硫酸酯的吸收特性。方法采用分子排阻凝胶色谱法、离子高效液相色谱法检测海参硫酸多糖高温高压降解前后分子量、硫酸根含量的变化,并利用PMP柱前衍生-高效液相色谱法测定岩藻聚糖硫酸酯的单糖组成,以及大鼠血清中单糖的变化。结果口服低分子量海参岩藻聚糖硫酸酯后,大鼠血清中岩藻糖和半乳糖的吸收速度和最大浓度明显高于中分子量岩藻聚糖组,血清中甘露糖、氨基葡萄糖的含量也显著上升,血清中氨基半乳糖的含量略有上升。而口服中、低分子量岩藻聚糖硫酸酯都能够降低血清中葡萄糖的含量。结论分子量低于10 kDa的低分子量岩藻聚糖具有很好的体内吸收率,适合于开发口服岩藻聚糖功能产品。Objective To investigate the influence of molecular weight of fucoidan from sea cucumber on the absorption and monosaccharide composition in serum in vivo,two fucoidan with different molecular weight from sea cucumber were prepared by hydrothermal degradation.Methods High performance molecular exclusion gel chromatography and high performance anion-exchange chromatography were used to analyze the molecular weight,sulfate content and monosaccharide compositionof fucoidan.The monosaccharide composition and the absorption of fucoidan in rat serum were determined by PMP pre-column derivatization-high performance liquid chromatography.Results After oral administration of low molecular weight fucoidan,the absorption rate and concentration of fucose and galactose in rat serum were significantly higher than those in medium molecular weight group.Meanwhile,oral administration of low molecular weight fucoidan could significantly increase the content of mannose and glucosamine in serum and slightly increase the content of galactosamine in serum.Oral administration of medium and low molecular weight sea cucumber fucoidan could reduce the content of glucose in the serum.Conclusion The low molecular weight fucoidan with molecular weight lower than 10 kDa,was easier to be absorbed and was a good choice for the oral fuctional drug.

关 键 词：海参 硫酸多糖 高温高压降解 吸收 

分 类 号：R914[医药卫生—药物化学]