表没食子儿茶素没食子酸酯和维生素C联用对高尿酸血症小鼠血尿酸水平的影响  

The Intergative Effects of Epigallocatechin-3-gallate and Vitamin C on Serum Uric Acid Levels in Hyperuricemic Mice

作　　者：徐燕 蔡吓强[1,2] 解千金 邰玲玲 刘增辉 XU Yan;CAI Xiaqiang;XIE Qianjin;TAI Lingling;LIU Zenghui(State Key Laboratory of Tea Biology and Utilization,Hefei 230036,China;School of Tea&Food Science and Technology,Anhui Agricultural University,Hefei 230036,China;Anhui Academy of Medical Science,Hefei 230061,China)

机构地区：[1]茶树生物学与资源利用国家重点实验室,安徽合肥230036 [2]安徽农业大学茶与食品科技学院,安徽合肥230036 [3]安徽省医学科学研究院,安徽合肥230061

出　　处：《茶叶科学》2020年第3期407-414,共8页Journal of Tea Science

基　　金：安徽省自然科学基金(1908085MC74);安徽省重点研究与开发计划项目(202004h07020003);安徽省茶叶化学与健康领军人才团队引进重大示范项目。

摘　　要：以KM雄性小鼠为研究对象,酵母膏(7.5 g·kg^-1)和氧嗪酸钾(250 mg·kg^-1)联合给药建立高尿酸血症小鼠模型,探究表没食子儿茶素没食子酸酯(EGCG)和维生素C(Vc)联用对高尿酸血症小鼠血尿酸水平的影响。将小鼠随机分为6组(n=6):空白组、模型组、别嘌呤醇组(阳性药组)、EGCG组、EGCG联合Vc组和Vc组,连续给药7 d后测定生化指标。结果表明,与模型组相比,EGCG联合Vc组小鼠的血尿酸值(UA),血尿素氮(BUN)和肌酐(Cr)水平均明显降低(P<0.001);EGCG与Vc联用明显抑制了肝脏中腺苷脱氨酶(ADA)和黄嘌呤氧化酶(XOD)的活性(P<0.05或P<0.01),并显著下调了肾脏中葡萄糖转运蛋白9(GLUT9)mRNA的表达(P<0.001);肾脏切片显示EGCG和Vc联用显著改善高尿酸血症小鼠的肾脏损伤。此外,EGCG与Vc联用对高尿酸血症小鼠的作用效果优于EGCG。KM male mice were used as subjects of the study.Yeast extracts(7.5 g·kg^-1)and potassium oxonate(250 mg·kg^-1)were administered to establish the hyperuricemic mice model.The study aimed to investigate the integrative effect of EGCG and vitamin C(Vc)on serum uric acid levels of hyperuricemic mice.Mice were randomly divided into 6 groups(n=6):blank group,model group,allopurinol group,EGCG group,EGCG+Vc group and Vc group.The biochemical indexes of mice were measured after 7 d of continuous administration.The results show that the serum uric acid(UA),serum urea nitrogen(BUN)and creatinine(Cr)of EGCG+Vc group were significantly lower than those of the model group(P<0.001).The combination of EGCG and Vc could remarkably inhibited the activities of adenosine deaminase(ADA)and xanthine oxidase(XOD)in the liver(P<0.05 or P<0.01)and significantly down-regulated the expression of glucose transporter 9(GLUT9)in the kidney(P<0.001).The results of kidney slices indicated that EGCG+Vc could obviously improve the damages to the kidney in hyperuricemic mice.In addition,the integrative effect of EGCG+Vc on hyperuricemic mice was better than that of EGCG.

关 键 词：表没食子儿茶素没食子酸酯 维生素C 高尿酸血症 血尿酸 葡萄糖转运蛋白9 

分 类 号：S571.1[农业科学—茶叶生产加工] R983+.2[农业科学—作物学]