铁观音茶末多糖的分离纯化和抗氧化活性  

Isolation,Purification and Antioxidant Activity of Polysaccharide from Tieguanyin Tea Dust

作　　者：吴金松 张岩[1] 陈晓培 耿广威 丁德刚[1] 徐军[1] 王保营 WU Jin-song;ZHANG Yan;CHEN Xiao-pei;GENG Guang-wei;DING De-gang;XU Jun;WANG Bao-ying(Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China)

机构地区：[1]河南牧业经济学院,河南郑州450046

出　　处：《食品工业科技》2020年第11期66-71,共6页Science and Technology of Food Industry

基　　金：省级院级重点学科建设校企合作项目(功能复合包装材料503/14020813)。

摘　　要：为实现废弃茶叶资源的再利用以及探究铁观音茶叶末活性多糖的抗氧化活性,以水提醇沉法提取铁观音茶末粗多糖,然后通过DEAE-52纤维素和Sephadex-100葡聚糖凝胶分级两次纯化,并进行纯度鉴定、红外光谱分析、分子量的测定以及体外抗氧化活性的测定等。结果表明,90%的乙醇终浓度得率最高,为1.97%;两次分级纯化后得到TPS-1A和TPS-4C,纯度分别为96.2%、95.1%;红外光谱图谱表明两种多糖均为β-型糖苷键多糖;分子量分别为15792、21722 Da;两种多糖组分抗氧化活性均随着组分浓度的增加而逐渐增强,当质量浓度为1.2 mg/mL时TPS-1A和TPS-4C,对DPPH自由基的清除率分别为95.41%、97.71%,羟自由基清除率分别为93.39%、94.21%,总还原力测得吸光度值为0.699、0.712。由此说明铁观音茶末多糖具有较强的抗氧化活性,且TPS-4C强于TPS-1A。To achieve the reuse of the waste tea resources,and to explore the antioxidant activity of Tieguanyin tea at the end of the activity of polysaccharide,the extraction of crude polysaccharide from Tieguanyin tea was optimized by water extraction and alcohol precipitation and then by DEAE cellulose and Sephadex-G100-glucan gel grade two purification,and the purity identification,infrared spectroscopy,the determination of molecular weight as well as the determination of antioxidant activity in vitro were determined.The results showed that the yield of 90%ethanol was 1.97%.TPS-1 A and TPS-4 C were obtained after two times of fractional purification and the purity was 96.2%and 95.1%,respectively.Infrared spectrum showed that both polysaccharides were beta glycoside bond polysaccharides.The molecular weights were 15792 and 21722 Da,respectively.The antioxidant activity of TPS-1 A and TPS-4 C increased gradually with the increase of component concentration.When the mass concentration was 1.2 mg/mL,the scavenging rate of DPPH radical was 95.41%and 97.71%,and the hydroxyl radical scavenging rate was 93.39%and 94.21%,respectively.The absorbance value of the total reducing force was 0.699 and 0.712.This indicated that Tieguanyin tea powder polysaccharide had strong antioxidant activity,and TPS-4 C was stronger than TPS-1 A.

关 键 词：铁观音茶末多糖 分离纯化 红外光谱扫描 分子量测定 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]