青钱柳叶抗氧化物质积累的差异性研究  

Comparison study on antioxidant compounds accumulation in Cyclocarya paliurus leaves

作　　者：郑晓杰[1,2] 林胜利 吴聪聪[3] 聂小华 ZHENG Xiaojie;LIN Shengli;WU Congcong;Nie Xiaohua(Wenzhou College of Technology and Profession(Wenzhou Academy of Agricultural Sciences),Wenzhou 325006,China;Institute of Modern Agriculture and Health Industry of Wencheng County,Wenzhou 325006,China;College of Food Science and Technology,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]温州科技职业学院(温州市农业科学研究院),浙江温州325006 [2]文成县现代农业与康养产业研究院,浙江温州325006 [3]浙江工业大学食品科学与工程学院,浙江杭州310014

出　　处：《食品与发酵工业》2020年第11期238-243,共6页Food and Fermentation Industries

基　　金：浙江省重点研发计划(2017C02039);文成县创新创业种子资金项目(2018NKY02);温州市重点实验室(工程中心)建设项目(ZD202003)。

摘　　要：为探究青钱柳叶适宜采收期,以浙江文成(WC)和湖南张家界(ZJJ)同种源5年生青钱柳为对象,研究其嫩叶抗氧化活性与酚类物质积累的动态变化。结果表明,随着采收期延迟,WC青钱柳叶自由基清除能力、还原力、总酚含量和总黄酮含量呈先增后降趋势,8月均达到峰值;而ZJJ青钱柳叶抗氧化活性和总酚含量表现出增加-降低-增加的双峰特征,除9月外总黄酮含量未有明显变化。在青钱柳叶提取物中初步鉴定出2种酚酸和10种黄酮,其中(+)-儿茶素仅在WC青钱柳叶中检出;2,3-O-二咖啡酰酒石酸含量与抗氧化活性有着高度正相关(r=0.917~0.941)。这说明青钱柳叶适宜采收期因产地而异,2,3-O-二咖啡酰酒石酸在其抗氧化活性中起到较为重要的作用。In order to explore the appropriate harvest time,the variation of antioxidant capacity and phenolic compounds in the leaves from 5-year-old C.paliurus trees of the same cultivar across two cultivation sites(Wencheng,WC;Zhangjiajie,ZJJ)were investigated.DPPH and ABTS scavenging ability,reducing power,total phenolic and flavonoid contents of WC leaves increased initially then decreased as the season progressed.Whereas antioxidant capacity and total phenolic content showed a distinct bimodal pattern,along with a slight variation in total flavonoid content except for September.Two phenolic acid and ten flavonoids were qualitatively identified in the extracts of C.paliurus leaves.Among them,(+)-catechin was detected in WC leaves,but not in ZJJ leaves.And the content of 2,3-O-dicaffeoyl tartaric acid was highly positively correlated with the tested antioxidant capacities(r=0.917-0.941).The results suggested that the appropriate harvest time of C.paliurus leaves,despite the same cultivar,differed across the cultivation site,and 2,3-O-dicaffeoyl tartaric acid might act as an important phenolics for antioxidant capacity of the eaves.It would provide the basis for quality control and product development of C.paliurus leaves.

关 键 词：青钱柳 采收期 抗氧化活性 酚类物质 2 3-O-二咖啡酰酒石酸 

分 类 号：S567.19[农业科学—中草药栽培]