褚橙精油纳米乳的制备及抑菌性  

Preparation and Antibacterial Activity of Chu Orange Essential Oil Nanoemulsion

作　　者：刘如楠 黄凯 管骁[1] 李森[1] 刘静[2] 宋洪东 LIU Runan;HUANG Kai;GUAN Xiao;LI Sen;LIU Jing;SONG Hongdong(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;College of Information Engineering,Shanghai Maritime University,Shanghai 200135,China)

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093 [2]上海海事大学信息工程学院,上海200135

出　　处：《食品与生物技术学报》2020年第5期59-67,共9页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31701515);国家粮食行业青年拔尖人才计划项目(LQ2018204)。

摘　　要：由于褚橙(Citrus sinensis Osbeck)精油特有的香气和抑菌性,使褚橙精油在食品、医药、化妆品等行业有着极其广泛的应用。但褚橙精油的水溶性差、且易挥发特性限制了其应用。为解决这些问题,以非离子表面活性剂(Tween 80和Span 80)为乳化剂,采用高速剪切的方法制备了褚橙精油O/W纳米乳液。并研究了表面活性剂复配比、质量浓度以及乳化剪切速率对褚橙精油纳米乳粒径、ζ-电位、黏度、乳液稳定性的影响。探索了制备褚橙精油的优化工艺参数:当褚橙精油的质量浓度为5 g/dL时,Tween 80和Span 80复配质量比为1∶2,复合表面活性剂质量浓度为3 g/dL,乳化剪切速度为12000 r/min,所得乳液粒径为(135±0.76)nm,ζ-电位绝对值(30±0.36)mV,稳定性良好。研究发现,相比较于纯精油,褚橙精油纳米乳表现出更强的抑菌效果,说明将精油乳化可以提高其利用效率,这为该精油的应用拓展提供了可能。Chu orange(Citrus sinensis Osbeck)essential oil is widely applied in food,medical,and cosmetic industries for its unique odor and antibacterial properties.However,its poor water solubility and strong volatility limit the wider application of this essential oil.In order to overcome these disadvantages,O/W Chu orange essential oil nanoemulsion was prepared with the non-ionic surfactant emulsifiers Tween 80 and Span 80.The effects of surfactant ratio,concentration and emulsification shear rate on the particle size,zeta potential,rheological viscosity and emulsion stability of Chu orange essential oil nanoemulsion were studied.The stable nanoemulsion with the particle size(135±0.76)nm and the absolute vale of zeta potential(30±0.36)mV was obtained under the conditions of 5%(w/w)Chu orange oil,3%Tween 80 and Span 80(1∶2),and shear rate of12000 r/min.In addition,the antibacterial activity of the Chu orange essential oil nanoemulsion was stronger than that of the pure essential oil,which suggested that nanoemulsion can efficiently improve its utilization.This opens up the possibility of expanding the application of Chu orange essential oil.

关 键 词：褚橙精油 纳米乳 稳定性 抑菌性 

分 类 号：Q592.6[生物学—生物化学]