Links between donor macrosteatosis,interleukin-33 and complement after liver transplantation  

作　　者：Kelley Núñez Mohammad Hamed Daniel Fort David Bruce Paul Thevenot Ari Cohen 

机构地区：[1]Institute of Translational Research,Ochsner Clinic Foundation,New Orleans,LA 70121,United States [2]Center for Outcomes and Health Services Research,Research Administration,Ochsner Clinic Foundation,New Orleans,LA 70121,United States [3]Multi-Organ Transplant Program,Ochsner Clinic Foundation,New Orleans,LA 70121,United States

出　　处：《World Journal of Transplantation》2020年第5期117-128,共12页世界移植杂志

摘　　要：BACKGROUND As prevalence of nonalcoholic fatty liver disease increases in the population,livers with steatosis will continue to infiltrate the donor pool.Safe utilization of these extended criteria grafts is paramount given the increased risk associated with their use in transplantation.Prognostic factors that can predict liver dysfunction immediately after transplantation with macrosteatotic grafts are lacking.AIM To understand the relationship between interleukin-33(IL-33)and complement in recipients immediately following liver reperfusion as a marker of liver dysfunction.METHODS Cohort consisted of patients who received a liver transplant from September 2016–September 2019 at our institution.Clinical variables were retrospectively extracted from the electronic medical record.Back-table donor biopsies were obtained with donor steatosis percentage retrospectively determined by a boardcertified pathologist.Blood samples were available immediately following liver transplantation.Quantification of plasma IL-33 and complement proteins,C3a and C5a,were determined by enzyme-linked immunosorbent assay.For mRNA expression,RNA was extracted from donor biopsies and used against a 780 gene panel.RESULTS Cohort consisted of 99 donor and recipients.Donor median age was 45 years and 55%male.Recipients had a median age of 59 years with 62%male.The main etiologies were alcoholic hepatitis,nonalcoholic steatohepatitis,and hepatocellular carcinoma.Median MELD-Na at transplant was 21.Donors were grouped based on moderate macrosteatosis(≥30%).Recipients implanted with moderate macrosteatotic grafts had significantly higher peak alanine aminotransferase/aspartate aminotransferase(P<0.001 and P<0.004),and increased incidence of early allograft dysfunction(60%compared to 18%).Circulating IL-33 levels were significantly elevated in recipients of≥30%macrosteatotic grafts(P<0.05).Recipients with detectable levels of circulating IL-33 immediately following reperfusion had significantly higher alanine aminotransferase/aspartate amino

关 键 词：Liver transplantation INTERLEUKIN-33 Donor macrosteatosis COMPLEMENT Early allograft dysfunction REPERFUSION 

分 类 号：R57[医药卫生—消化系统]