响应面优化设计饮料专用酵母抽提物多糖提取工艺  

Response Surface Optimized the Extraction Technology of Polysaccharides from Yeast Extract for Beverage

作　　者：李凡 许琦 熊建[1] 李知洪[1] 姚鹃[1] 李沛[1] 潘思轶[2] 彭颖 LI Fan;XU Qi;XIONG Jian;LI Zhihong;YAO Juan;LI Pei;PAN Siyi;PENG Ying(Angel Yeast CO.,LTD.,Yichang 443003,China;College of Food Science&Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]安琪酵母股份有限公司研发中心,湖北宜昌443003 [2]华中农业大学食品科技学院,湖北武汉430070

出　　处：《食品科技》2020年第4期164-168,共5页Food Science and Technology

基　　金：2019年湖北省博士后创新岗位基金项目;国家自然科学基金项目(31571847)。

摘　　要：为了研究饮料专用酵母抽提物中多糖的提取条件及含量,文章通过单因素和响应面试验对饮料专用酵母抽提物(YE-B)中多糖的乙醇浸提工艺条件进行了优化。结果表明,YE-B中多糖的最佳提取工艺参数为乙醇体积分数68%、浸提温度40 ℃、液料比16:1,此时多糖的含量为27.08%,接近响应面预测值,因此响应面优化YE-B的乙醇浸提工艺真实可信。经检测,YE-B含有26.8%的β-葡聚糖及8.70%的甘露寡糖,表明YE-B具有很好的生物活性。In order to study the extraction conditions and content of polysaccharides in beverage-specific yeast extracts,in this essay the conditions of ethanol extraction of polysaccharides in yeast-specific yeast (YE-B) were optimized by single factor and response surface experiments.The results showed that the optimal extraction process parameters of polysaccharides in YE-B were 68% ethanol volume fraction,40 ℃ extraction temperature,and 16:1 liquid-to-material ratio.At this time,the polysaccharide content was 27.08%,which was close to the predicted value of the response surface,so that the ethanol extraction process of YE-B was true and credible.According to the test,YE-B contains 26.8% beta-glucan and 8.70% manna oligosaccharide,so YE-B has good biological activity.

关 键 词：响应面优化法 饮料专用 酵母抽提物 多糖 提取工艺 

分 类 号：R284.2[医药卫生—中药学]