植物源重组人血清白蛋白对BALB/c小鼠的致敏性评价  

Assessment of allergenicity of oryza sativa recombinant human serum albumin in BALB/c mice

作　　者：杨柳 陈晨 韩超 赵金鹏 刘婷婷 石丽丽 宫照龙 沈葹 卓勤 Yang Liu;Chen Chen;Han Chao;Zhao Jinpeng;Liu Tingting;Shi Lili;Gong Zhaolong;Shen Shi;Zhuo Qin(National Institute for Nutrition and Health,Chinese Center for Disease Control and Prevention,Beijing 100050,China)

机构地区：[1]中国疾病预防控制中心营养与健康所,北京100050

出　　处：《卫生研究》2020年第3期463-466,共4页Journal of Hygiene Research

基　　金：转基因生物新品种培育重大专项(No.2016ZX08011005)。

摘　　要：目的利用BALB/c小鼠,探讨植物源重组人血清白蛋白(oryza sativa recombinant human serum albumin,OsrHSA)是否具有潜在致敏性。方法将80只BALB/c小鼠按体重随机分为4组,分别为卵清蛋白阳性对照组、马铃薯酸性磷酸酶(potato acid phosphatase,PAP)阴性对照组、OsrHSA组和磷酸盐缓冲液溶剂对照组,每组20只。分别于第0、3、6、9和12 d给小鼠腹腔注射0.2 mg/mL相应蛋白溶液。酶联免疫法检测血浆组胺水平和血清中特异性IgE抗体(sIgE)、特异性IgG抗体(sIgG)、特异性IgG1抗体(sIgG1)、特异性IgG2a抗体(sIgG2a)及总IgE抗体(tIgE)水平。结果卵清蛋白组血清tIgE、sIgE、sIgG、sIgG1和血浆组胺含量显著高于其他各组,而血清sIgG2a含量降低,差异有统计学意义(P<0.05)。OsrHSA组血清sIgE水平和组胺水平与溶剂对照组相比差异无统计学意义(P>0.05),血清sIgG、sIgG1和sIgG2a含量低于PAP组(P<0.05),而血清tIgE含量与PAP组相比差异无统计学意义(P>0.05)。结论腹腔注射OsrHSA对BALB/c小鼠的潜在致敏性很低。OBJECTIVE To investigate the potential allergenicity of oryza sativa recombinant human serum albumin(OsrHSA)in BALB/c mice.METHODS Eighty BALB/c mice were randomly divided into four groups i.e ovalbumin(OVA)positive control group,potato acid phosphatase(PAP)negative control group,Oryza sativa recombinant HAS(OsrHSA)group and solvent control group(phosphate buffer saline,PBS),respectively.Mice were administered by intraperitoneal injections of tested proteins and histamine levels in plasma and sIgE,sIgG,sIgG1,sIgG2 a,and tIgE antibody levels in serum were measured.RESULTS Compared with the other groups,serum tIgE,sIgE,sIgG,sIgG1 and plasma histamine levels in the OVA group were significantly increased,while serum sIgG2 a levels were decreased(P<0.05).There was no significant difference in serum sIgE level and histamine level between the OsrHSA group and the control group(P>0.05).Serum sIgG,sIgG1 and sIgG2 a levels were lower than those in the PAP group(P<0.05).There was no significant difference in serum tIgE content between PAP group and OsrHSA group(P>0.05).CONCLUSION The potential allergenicity of OsrHSA through traperitioneal injection in BALB/c mice was very low.

关 键 词：BALB/C小鼠 人血清白蛋白 致敏性 转基因食品 食品安全 

分 类 号：R155.51[医药卫生—营养与食品卫生学] TS201.6[医药卫生—公共卫生与预防医学]