桂花种子油的抗氧化活性和成分测定  

Antioxidant Activity and Composition of the Osmanthus Fragrans Seeds Oil

作　　者：陈晓凤 刘刚[1,2] 李学理[1] 汪淑芳[1,2] 张晓喻[1,2] 赵甲元 CHEN Xiaofeng;LIU Gang;LI Xueli;WANG Shufang;ZHANG Xiaoyu;ZHAO Jiayuan(College of Life Sciences,Sichuan Normal University,Chengdu 610101,Sichuan;Food Function and Application Research Institute,Sichuan Normal University,Chengdu 610101,Sichuan)

机构地区：[1]四川师范大学生命科学学院,四川成都610101 [2]四川师范大学食品功能及加工应用研究所,四川成都610101

出　　处：《四川师范大学学报（自然科学版）》2020年第4期544-549,共6页Journal of Sichuan Normal University（Natural Science）

基　　金：四川省教育厅重点项目(17ZB0353);四川省大学生创新创业训练计划项目(201610636120)。

摘　　要：为开发利用桂花种子油,以石油醚为溶剂超声波辅助提取油脂,分别用DPPH法和ABTS法测定桂花种子油的抗氧化活性,用气相色谱法分析其脂类成分的含量.结果表明,桂花种子油对ABTS和DPPH自由基有显著的清除效果,IC50值分别为271. 963和286. 347 mg/m L.桂花种子油的质量浓度在62. 500~500. 000 mg/m L范围,清除率在38. 460%~89. 840%,最高可达90. 000%. GC法测定桂花种子油,结果表明有8种主要成分,其中,油酸42. 600%、亚油酸40. 900%、棕榈酸11. 200%和硬脂酸3. 030%,这4种主要成分占96. 000%.桂花种子油中,饱和脂肪酸、单不饱和脂肪酸与多不饱和脂肪酸的比例,比较接近最佳食用脂肪酸的比例.因此,桂花种子油属于高亚油酸型的植物油,具有抗氧化功能,具有开发成功能食品的价值.Summarizing the biological characteristics and research advance of Osmanthus fragrans by looking up literature,select the seeds of O.fragrans as material.The seeds oil of O.fragrans fruits was extracted by the light pertroleum as its solvent with ultrasonic wave as assistant.The antioxidant activities of the oils in vitro were evaluated by scavenging capacity against ABTS and DPPH.Their chemical components were analyzed by Gas chromatography(GC).The results showed that the seeds oil exhibited significant antioxidant activities in vitro in a concentration-dependent fashion.The IC50 of scavenging capacity against ABTS and DPPH were 271.963 mg/mL and 286.347 mg/mL,respectively.It was found experimentally that the seed oil had strong hydroxyl free radical scavenging activity when the concentration is from 62.500 mg/mL to 500.000 mg/mL,its scavenging activity maintained at 38.460%~89.840%,and it had a better scavenging hydroxyl free radical clearance rate reaching 90.000%.There are 8 main compounds in the oils,and 4 main compounds in the oils occupied 96%of total constituents.The 4 compounds were oleic acid,linoleic acid,palmitic acid and stearic acid,which occupied 42.600%,40.900%,11.200%and 3.030%of total constituents,respectively.Oleic acid was polyunsaturated fatty acids and linoleic acid was monounsaturated,palmitic acid and stearic acid were saturated fatty acids in the oil.O.fragrans seed oil is high linoleic acid type vegetable oil which has certain development value.

关 键 词：桂花 种子油 抗氧化活性 气相色谱 成分测定 

分 类 号：S567.1[农业科学—中草药栽培] Q599[农业科学—作物学]