不同分子量蛤类多肽的抗氧化活性研究  

Study on Antioxidant Activity of Different Molecular Weight Segmens of Clam Polypeptides

作　　者：穆姣姣 赵前程[2] 崔相勇 李建伟[2] 李智博[2] Mu Jiaojiao;Zhao Qiancheng;Cui Xiangyong;Li Jianwei;Li Zhibo(Shenyang Institute for Food and Drug Control,Shenyang 110000,China;College of Food Engineering,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]沈阳市食品药品检验所,辽宁沈阳110000 [2]大连海洋大学食品工程学院,辽宁大连116023

出　　处：《现代食品》2020年第10期81-83,共3页Modern Food

摘　　要：本文对美洲帘蛤和菲律宾蛤仔进行酶解制备了蛤蛋白酶解物,采用超滤技术将小于6000 Da的肽进行分离,以DPPH和羟自由基清除率作为抗氧化活性指标。研究结果表明:低分子量肽抗氧化活性较强,两种蛤蛋白酶解液超滤后的组分(小于6000 Da)抗氧化活性明显提高。美洲帘蛤DPPH自由基和羟自由基清除率分别是(89.13±0.77)%和(87.14±0.86)%;而菲律宾蛤仔分别为(45.73±0.60)%和(89.95±1.41)%。In this study,the Mercenaria mercenaria and Ruditapes philippinarum protein hydrolysates were prepared by enzymatic methods.The peptides whose molecular weight were less than 6000 Da were separated through ultrafiltration.Using DPPH radical and hydroxyl radical scavenging rate as antioxidant activity index.The results showed that:The antioxidant activity of low molecular weight peptide was higher.After ultrafiltration,two calm protein hydrolysates with molecular weight less than 6000 Da was highly improved.The DPPH radicals and hydroxyl radicals scavenging rate of Mercenaria mercenaria were(89.13±0.77)%and(87.14±0.86)%,respectively,and that of Ruditapes philippinarum were(45.73±0.60)%and(89.95±1.41)%,respectively.

关 键 词：美洲帘蛤 菲律宾蛤仔 蛋白酶解物 超滤 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]