Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy  

作　　者：ANDrea Krisztina Sükosd Krisztina Szabadfi Edina Szabó-Meleg Beáta Gáspár Pavel Stodulka Gyorgy SétálóJr Róbert Gábriel Miklós Nyitrai Zsolt Biró Hajnalka Abrahám 

机构地区：[1]Department of Ophthalmology,University of Pecs Medical School and Clinical Centre,Pecs 7623,Hungary [2]Department of Experimental Zoology and Neurobiology,University of Pecs,Pecs 7624,Hungary [3]Department of Biophysics,University of Pecs Medical School and Clinical Centre,Pecs 7624,Hungary [4]Janos Szentagothai Research Centre,University of Pecs,Pecs 7624,Hungary [5]Optimum Laser Centre,Budapest 1124,Hungary [6]Gemini Eye Clinics,Zlin 76001,Czech Republic [7]Department of Medical Biology and Central Electron Microscopic Laboratory,University of Pecs Medical School,Pecs 7624,Hungary

出　　处：《International Journal of Ophthalmology(English edition)》2020年第6期927-934,共8页国际眼科杂志（英文版）

基　　金：Supported by EFOP-3.6.3-VEKOP-16-2017-00009 and GINOP-2.3.2-15-2016-00036 to University of Pécs Medical School;EFOP-3.6.1-16-2016-00004 project;The Higher Education Institutional Excellence Programme of the Ministry for Innovation and Technology in Hungary,within the framework of the 5.thematic programme of the University of Pécs。

摘　　要：AIM:To study the effect of mechanical stress on the cytoskeleton in lens epithelial cells following conventional phacoemulsification surgery(CPS)and femtosecond laserassisted cataract surgery(FLACS).METHODS:The cytoskeleton of the epithelial cells of the anterior lens capsules(ALC)removed by CPS and FLACS was examined by immunohistochemistry.Expression of the intermediate filament,glial fibrillary acidic protein(GFAP),and glutamine synthetase(GS)immunoreactivity were detected.In order to map the actin network of cells,fluorescently labeled phalloidin was used.The samples were examined using confocal laser scanning microscopy.RESULTS:GFAP expression was visible in a larger number of the epithelial cells after CPS compared to FLACS.In CPS sample’s epithelial cells,GFAP immunoreactivity indicated robust morphological change.Regarding the actin filaments,the presence of tubular elements connecting epithelial cells,regular actin pattern and marked cortical network after CPS were found.Following FLACS,the actin cytoskeleton of the epithelial cells remained densely structured,and the tubular elements were undetectable,however,the above-mentioned regular actin pattern and the marked cortical network were visible.CONCLUSION:The conventional removal of the ALC induces more robust changes of the cytoskeleton of the lens epithelial cells.

关 键 词：lens epithelial cell CYTOSKELETON CAPSULOTOMY glial fibrillary acidic protein ACTIN 

分 类 号：R779.6[医药卫生—眼科]