药用真菌对山药基质的双向发酵适应性评价  

Bidrectional-fermentation Adaptability of Medicinal Fungal on Chinese Yam

作　　者：李慧星[1,2] 许彬 李斌[1,2] 郭书贤 李杰[1,2] Li Huixing;Xu Bin;Li Bin;Guo Shuxian;Li Jie(College of Biology and Chemistry Engineering,Nanyang Institute of Technology,Nanyang 473004,Henan;Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology,Nanyang 473004,Henan)

机构地区：[1]南阳理工学院生物与化学工程学院,河南南阳473004 [2]河南省工业微生物资源与发酵重点实验室,河南南阳473004

出　　处：《中国食品学报》2020年第6期188-193,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：河南省2017科技攻关计划项目(172102110109)。

摘　　要：以山药为药用真菌的培养基质进行双向发酵,评价药用真菌对山药基质的适应性。从10种药用真菌中选择能在山药基质上蔓延生长的菌株,以布满时间、折干率、消耗率、羟自由基清除率、DPPH自由基清除率、超氧自由基清除率为指标,利用主成分分析评价药用真菌对山药基质的适应性。结果表明:提取的2个主成分反映原变量的89.36%的信息。第1个主成分F1为双向发酵适应性因子,第2主成分为双向发酵耗时因子。结合主成分得分图,灵芝(14025)、香菇(14035)、蛹虫草(14013)和银耳(50179)对山药基质具有良好的适应性。灵芝对山药基质的适应性最好,然而发酵耗时最长。银耳和蛹虫草在山药基质上生长迅速,所得菌质具有较高的抗氧化活性。The Chinese yam was as solid-state fermentation matrix for bidrectional-fermentation using medicinal fungi.The adaptability of medicinal fungal on Chinese yam was evaluated.The strain which could grow and spread on Chinese yam matrix was chosen from 10 medicinal fungal strains.The bestrewing time,dry matter content,decomposition rate,scavenging rate of hydroxyl radical,DPPH,superoxide anion were analyzed.The adaptability of medicinal fungal on Chinese yam was evaluated by principal component analysis(PCA).The results showed that the first two components represented 89.36%of the total variability on the base of the total variance explained.The first principal component F1 was bidrectional-fermentation adaptability factor,the second principal component F2 was fermentation time-consuming factor.The sample score plots visually displayed that Ganoderma lucidium(14025),Lentinula sp.(14035),Cordyceps militaris(14013)and Tremella fuciformis(50179)had good adaptation on Chinese yam.The adaptation of Ganoderma lucidium(14025)on Chinese yam was the best,which also need long time to complete the fermentation.Cordyceps militaris(14013)and Tremella fuciformis(50179)grow fast,and the fungi substance also had better antioxidant activity.

关 键 词：双向发酵 药用真菌 山药 适应性 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]