响应面优化超声辅助法提取拐枣种子中二氢杨梅素的工艺  

Optimization of Ultrasound-assisted Extraction of Dihydromyricetin from Seeds of Hovenia dulcis Thunb.Using Response Surface Methodology

作　　者：张晓南 朱鸿维[2] 赵善舶 白晨 石雅楠 徐浩朋 尹恋 胡淑敏 尹花 ZHANG Xiao-Nan;ZHU Hong-Wei;ZHAO Shan-Bo;BAI Chen;SHI Ya-Nan;XU Hao-Peng;YIN Lian;HU Shu-Min;YIN Hua(State key laboratory of beer biological fermentation engineering,Qingdao beer co.,LTD.,Qingdao 266071;Food Science College,Northeast Agricultural University,Harbin 150036)

机构地区：[1]青岛啤酒股份有限公司,啤酒生物发酵工程国家重点实验室,青岛266071 [2]东北农业大学食品学院,哈尔滨150036

出　　处：《植物研究》2020年第5期775-781,共7页Bulletin of Botanical Research

基　　金：黑龙江省自然基金面上项目(C2018025)。

摘　　要：二氢杨梅素和杨梅素是拐枣种子中的重要成分。通过超声辅助法从拐枣种子中提取二氢杨梅素,采用单因素法考察乙醇体积分数、超声辐照功率、提取温度、液料比和超声辐照时间影响参数的基础上,并选用Box-Behnken响应面设计法分析建立了超声辐照功率、超声辐照时间和液料比的二次多项式模型,优化提取工艺。结果得到超声辅助法提取拐枣种子中二氢杨梅素的最佳工艺参数为:乙醇体积分数为60%、超声辐照功率140 W、超声辐照时间30 min、液料比20.5 mL·g-1,提取温度40℃。在此最佳条件下,二氢杨梅素得率为2.14±0.09 mg·g-1。本提取方法简单快速,效率高,有利于拐枣资源的综合加工利用。Dihydromyricetin is an important component in the seeds of Hovenia dulcis Thunb.The extraction of dihydromyricetin from seeds of H.dulcis by ultrasound-assisted method was carried out on the basis of single factor investigation of ethanol volume fraction,ultrasound irradiation power,extraction temperature,liquid-solid ratio and ultrasound irradiation time.The Box-Behnken response surface design method was used to establish a quadratic polynomial model of ultrasound irradiation power,ultrasound irradiation time and liquid-solid ratio,and optimized extraction process.The optimum conditions for the extraction were as follows:ethanol volume fraction of 60%,ultrasonic irradiation power of 140 W,ultrasonic irradiation time of 30 min,liquid-solid ratio of[JP3]20.5 mL·g-1,and extraction temperature of 40℃.Under these optimal conditions,the yield of[JP]dihydromyricetin was 2.14±0.09 mg·g-1.The extraction method is simple,rapid,and high in efficiency,which is beneficial to the comprehensive processing and utilization of the H.dulcis resources.

关 键 词：拐枣 种子 超声辅助提取 二氢杨梅素 响应面 

分 类 号：TQ423[化学工程]