原花青素对铁超载大鼠肾脏铁含量、氧化应激及Fas、Bax基因表达的影响  

Effects of Grape Seed Proanthocyanidins on Renal Iron Content,Oxidative Stress and Fas and Bax Gene Expression in Rats with Iron Overload

作　　者：云少君[1] 褚东阳 何兴帅 张文芳[1] 冯翠萍[1] YUN Shaojun;CHU Dongyang;HE Xingshuai;ZHANG Wenfang;FENG Cuiping(College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《食品科学》2020年第13期106-111,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目(31801551);山西省高等学校科技创新项目(2016150);山西农业大学引进博士科研启动费项目(2013YJ30)。

摘　　要：目的:以铁含量、氧化应激状态、肌酐和尿素氮水平、病理切片以及Fas、Bax基因的表达量为观测点,探究葡萄籽原花青素(grape seed proanthocyanidins,GSPAs)对铁超载大鼠肾脏功能的营养干预作用。方法:40只雄性SD大鼠随机分为对照组(NC组)、铁超载模型组(Model组)、原花青素组(GSPAs组)和实验组(Test组)。通过全自动血液分析仪检测红细胞(red blood cell,RBC)和血红蛋白(hemoglobin,Hb)水平;以电感耦合等离子体质谱仪测定肾脏组织中Fe含量;采用相应试剂盒测定血清总铁结合力(total iron binding capacity,TIBC)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、超氧化物歧化酶(superoxide dismutase,SOD)活力、丙二醛(malondialdehyde,MDA)含量、总抗氧化力(total antioxidant activity,T-AOC)、肌酐(creatinine,CR)和尿素氮(urea nitrogen,BUN)水平;苏木精-伊红(hematoxylin-eosin,HE)染色检测肾脏组织切片;实时荧光定量聚合酶链式反应检测肾脏组织Fas与Bax基因的表达量。结果:相较于NC组,Model组大鼠RBC数目、Hb水平、肾脏中Fe含量、MDA含量、CR和BUN水平、Fas及Bax基因表达量显著升高(P<0.05或P<0.01),而TIBC、GSH-Px及SOD活力显著降低(P<0.05或P<0.01),T-AOC差异不显著(P>0.05)。GSPAs组Hb水平、T-AOC显著升高,Fas基因表达量显著下调(P<0.05或P<0.01)。与Model组相比,Test组RBC、Hb、MDA、CR、BUN水平及Fas、Bax基因表达量显著降低(P<0.05或P<0.01);TIBC、GSH-Px活力、SOD活力、T-AOC显著升高(P<0.05或P<0.01)。结论:GSPAs能够对铁过量引起的大鼠肾脏铁超载、氧化损伤、生理功能紊乱及细胞凋亡起到保护作用。Objective:The effects of grape seed proanthocyanidins(GSPAs)on renal function in iron-overload rats were investigated by observing the iron content,oxidative stress status,creatinine and urea nitrogen levels,pathological sections and expression of Fas and Bax genes.Methods:Forty male SD rats were randomly divided into normal control group(NC),iron overload model group(model,injected with 100 mg/kg mb of iron dextran every other day),GSPAs group(gavaged at 100 mg/kg mb on a daily basis)and GSPA+iron overload group.Red blood cell(RBC)count and hemoglobin(Hb)levels were measured by an automatic blood biochemical analyzer,and iron content in kidney tissue was determined by inductively coupled plasma mass spectrometry(ICP-MS).Serum total iron binding capacity(TIBC),glutathione peroxidase(GSH-Px)activity,superoxide dismutase(SOD)activity,malondialdehyde(MDA)concentration,total antioxidant capacity(T-AOC),creatinine(CR)and urea nitrogen(BUN)levels were measured by commercial kits.Kidney sections were examined histologically by hematoxylin-eosin(HE)staining.Reverse transcription real-time polymerase chain reaction was used to determine the expression of Fas and Bax genes in kidney tissues.Results:Compared with the NC group,the RBC count,Hb level,renal Fe content,MDA content,CR and BUN level,Fas and Bax gene expression in the model group were significantly increased(P<0.05 or P<0.01),while TIBC,GSH-Px activity and SOD activity were significantly decreased(P<0.05 or P<0.01).There was no significant difference in T-AOC between the two groups(P>0.05).The Hb content and T-AOC in the GSPAs group were increased significantly,while the expression of the Fas gene was downregulated significantly(P<0.05 or P<0.01).Compared with the model group,the RBC number,Hb content,MDA content,CR and BUN levels,and Fas and Bax gene expression in the GSPA+iron overload group were significantly decreased(P<0.05 or P<0.01),while TIBC,GSH-Px activity,SOD activity and T-AOC were significantly increased(P<0.05 or P<0.01).Conclusion:Grape seed proanth

关 键 词：葡萄籽原花青素 铁负荷 肾脏 氧化应激 凋亡 

分 类 号：TS201.4[轻工技术与工程—食品科学]