微生物代谢工程在花色苷生产过程中的应用现状和前景  

Present and Future Application of Microbial Metabolic Engineering in Anthocyanin Production

作　　者：李跃 李国瑞[2] 陈永胜[1,2] LI Yue;LI Guorui;CHEN Yongsheng(College of Food Science,Shenyang Agricultural University,Shenyang 110866,China;College of Life Science,Inner Mongolia University for Nationalities,Tongliao 028000,China)

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110866 [2]内蒙古民族大学生命科学学院,内蒙古通辽028000

出　　处：《食品科学》2020年第13期260-266,共7页Food Science

基　　金：国家自然科学基金地区科学基金项目(31460353);内蒙古自治区科技创新引导资金项目(KJCX15002);内蒙古民族大学科学研究项目(MDK2016008;MDK2017034;MDK2017035);草原英才创新团队项目;国家自然科学基金青年科学基金项目(31401418)。

摘　　要：随着微生物代谢工程技术在合成生物学等领域的广泛应用,该技术已被用于提取多种植物天然产物。花色苷是一种水溶性天然色素,能使花和果实着色,已被广泛应用于食品和化妆品行业。目前,花色苷主要从植物材料中提取,但是提取的花色苷具有纯度低和性质不稳定等缺点,因此利用微生物代谢工程技术替代传统方法提取花色苷具有重要意义。本文阐述了工程菌合成花色苷的研究进展,对酶的选择、运输调节、尿嘧啶核苷-5’-二磷酸葡萄糖供应量的调控以及工艺优化等方面进行综述。对花色苷合成代谢途径的了解将为实现微生物生产花色苷的产业化提供重要信息。Microbial metabolic engineering has seen wide application in synthetic biology and the extraction of naturally occurring phytochemicals. Anthocyanins, a class of water-soluble natural pigments, are primarily responsible for the coloration of terrestrial plants and fruits, and are widely used in the food and cosmetic industries. At present, anthocyanins are mainly extracted from plant materials, being characterized by low product purity and unstable nature. Therefore, microbial metabolic engineering is a significant alternative to the traditional extraction method to produce anthocyanins. Here, we review recent progress in the biosynthesis of anthocyanins using engineered bacteria with respect to enzyme selection, transportation regulation, regulation of uridine-5’-diphosphate glucose supply, and process optimization. Understanding of the anthocyanin biosynthetic pathway will provide important information for the industrialization of microbial anthocyanin production.

关 键 词：花色苷 微生物代谢 酶工程 代谢工程 

分 类 号：TS201.3[轻工技术与工程—食品科学]