正山小种精油大孔树脂吸附工艺优化及其抗氧化活性分析  

A study on the preparation of essential oil of Lapsang Souchong with macroporous resins and its antioxidant activity

作　　者：何理琴 王彩楠 刘志彬[1,2] 张晨 张雯[1,2] 倪莉 HE Li-qin;WANG Cai-nan;LIU Zhi-bin;ZHANG Chen;ZHANG Wen;NI Li(Institute of Food Science and Technology,Fuzhou University,Fuzhou,Fujian 350108,China;Fujian Center of Excellence for Food Biotechnology,Fuzhou,Fujian 350108,China)

机构地区：[1]福州大学食品科学技术研究所,福建福州350108 [2]福建省食品生物技术创新工程技术研究中心,福建福州350108

出　　处：《食品与机械》2020年第6期170-175,共6页Food and Machinery

基　　金：国家重点研发计划资助项目(编号:2016YFD0400801)。

摘　　要：通过大孔树脂吸附法制备正山小种精油,优化制备工艺,并对其挥发性香气成分和抗氧化活性进行分析。试验结果表明,最佳的精油制备工艺为:以LX-8大孔树脂作为吸附树脂,按1∶350(树脂湿重∶香气水,g/mL)比例在恒温摇床振荡器(25℃,转速200 r/min)吸附正山小种香气水的香气成分,吸附时间为180 min,然后使用95%乙醇,按1∶5(树脂湿重∶乙醇,g/mL)比例在恒温摇床振荡器(25℃,转速200 r/min)中对大孔树脂进行解吸附,解吸附时间为150 min。该条件下获得最高浓度的正山小种精油,产品具备典型的正山小种的香气特征,其香气成分浓度是香气水的约35倍,回收率约51%。气相色谱—质谱联用(GC-MS)分析吸附过程各阶段香气水的成分发现,正山小种的特征香气成分可以被LX-8大孔树脂吸附。该精油具有一定的抗氧化活性,其DPPH自由基和ABTS自由基清除活力分别为4μg/mL抗坏血酸当量与3μg/mL抗坏血酸当量。In the present study,the Lapsang Souchong essential oil was prepared by using macroporous resin,during which the preparation process was optimized.Furthermore,the volatile aroma components and antioxidant activity of the product were analyzed.The results showed that the optimal process was using LX-8 macroporous resin as the adsorption resin,and the aromatic components were absorbed in a constant temperature shaker(25℃,200 r/min)for 180 min.The ratio of resin wet weight and substrate was 1∶350(g/mL).Subsequently,macroporous resin was desorbed by using 95%ethanol in a constant temperature shaker(25℃,200 r/min)for 150 min.The ratio of resin wet weight to ethanol solution was 1∶5(g/mL).Under the control of this condition,the highest concentration of Lapsang Souchong essential oil was obtained.The aromatic components were concentrated 35 times,with the recovery rate approximate 51%.Gas chromatography-mass spectrometry(GC-MS)analysis indicated that most of the characteristic aroma components of Lapsang Souchong were adsorbed by LX-8 macroporous resin.Furthermore,the product was found to possess profound antioxidant activity.Its DPPH and ABTS free radical scavenging activity are 4 and 3μg/mL ascorbic acid equivalent,respectively.

关 键 词：正山小种精油 大孔树脂吸附法 香气成分 抗氧化活性 

分 类 号：O657.63[理学—分析化学] TQ658.1[理学—化学]