冠突散囊菌繁殖体提取物抑菌活性研究  

Antibacterial activity of propagule extracts of Eurotium cristatum

作　　者：肖晗汕 李滔滔 汤涵 陈怡 汪无忌 刘纯 胡治远 余松林[1,2] 文瑞明 刘石泉 XIAO Hanshan;LI Taotao;TANG Han;CHEN Yi;WANG Wuji;LIU Chun;HU Zhiyuan;YU Songlin;WEN Ruiming;LIU Shiquan(School of Materials and Chemical Engineering,Hunan City university,Yiyang 413000,China;Hunan Provincial Key Lab of Dark Tea and Jin-hua,Hunan City university,Yiyang 413000,China)

机构地区：[1]湖南城市学院材料与化学工程学院,湖南益阳413000 [2]黑茶金花湖南省重点实验室(湖南城市学院),湖南益阳413000

出　　处：《食品与发酵工业》2020年第14期65-69,共5页Food and Fermentation Industries

基　　金：黑茶金花湖南省重点实验室(2016TP1022);湖南省重点研发计划资助项目(2018NK2036);湖南省教育厅重点项目(19A094);微生物分子生物学湖南省重点实验开放基金(2019FW02);安化黑茶湖南省科学普及教育基地(湘科协通[2018]86号)。

摘　　要：为探讨冠突散囊菌繁殖体提取物的抑菌性能,该文采用抑菌圈测定不同繁殖体提取物对金黄色葡萄球菌、藤黄微球菌、大肠杆菌、枯草芽孢杆菌、白色链霉菌和白色念珠菌的抑菌活性,并探讨抑菌物质在不同温度、p H值和紫外光下的稳定性。该研究采用玻璃珠振摇法破碎冠突散囊菌繁殖体,获得子囊果、子囊孢子和分生孢子提取物。结果表明,冠突散囊菌子囊果和子囊孢子提取物有抑菌作用,分生孢子提取物无抑菌作用。其中,对藤黄微球菌、金黄色葡萄球菌和大肠杆菌的抑制作用最显著,且该物质在25~80℃,p H 2~6能保持稳定的抑菌活性。冠突散囊菌有性繁殖体(子囊果和子囊孢子)中有抑菌物质,抑菌物质在一定的p H值和温度范围内具有良好的稳定性。To investigate the antibacterial properties of propagule extracts of Eurotium cristatum,we determined the antibacterial activity of extracts from different Eurotium cristatum propagules( e. g.,ascocarps,ascospores,and conidia) against Staphylococcus aureus,Escherichia coli,Candida albicans,Streptomyces albus,Micrococcus luteus,and Bacillus subtilis using the disk diffusion test. Additionally,we explored the stability of their active antibacterial ingredients under different temperatures,duration of exposure to ultraviolet light,and pH. Eurotium cristatum propagules were disrupted using a glass bead-beating method to prepare the water extracts of ascocarps,ascospores,and conidia. The results suggested that the extracts of ascocarps and ascospores exerted significant antibacterial effects,primarily against M. luteus,S. aureus,and E. coli. Moreover,these extracts could maintain stable antibacterial activity at 25-80 ℃ and p H 2-6. However,the extract of conidia lacked any antibacterial effect. In conclusion,the sexual propagules( ascocarps and ascospores) of Eurotium cristatum contain active antibacterial ingredients that exhibit promising stability under certain pH and temperature ranges.

关 键 词：冠突散囊菌 子囊果 分生孢子 抑菌活性 稳定性 

分 类 号：TS272.5[农业科学—茶叶生产加工]