化橘红柚皮苷对α-葡萄糖苷酶活性的抑制作用  

The Inhibition Activity of α-Glucosidase by the Naringin from Exocarpium citri Grandis

作　　者：姜翠翠[1] 董舒梅 邱松山[1] 周英彪 JIANG Cuicui;DONG Shumei;QIU Songshan;ZHOU Yingbiao(Development Center of Technology for Fruit&Vegetable Storage and Processing Engineering,Guangdong University of Petrochemical Technology,Maoming 525000)

机构地区：[1]广东石油化工学院果蔬加工与贮藏工程技术开发中心,茂名525000

出　　处：《食品工业》2020年第6期189-193,共5页The Food Industry

基　　金：广东省科技计划项目(项目编号2018A030307051和2017A020225043);广东高校果蔬加工与贮藏工程技术开发中心开放基金(项目编号2012gczxB001)。

摘　　要：为探究化橘红柚皮苷对α-葡萄糖苷酶活性的抑制作用和清除DPPH自由基能力,采用体外α-葡萄糖苷酶抑制模型,测定化橘红柚皮苷对α-葡萄糖苷酶的抑制能力及清除DPPH自由基能力,并分析其对α-葡萄糖苷酶活性的抑制作用类型。结果表明,化橘红柚皮苷对α-葡萄糖苷酶活性的抑制作用优于阿卡波糖,柚皮苷主要以剂量依赖性、时间依赖性的方式抑制α-葡萄糖苷酶的活性,其对α-葡萄糖苷酶的半抑制浓度(IC50)为0.008 mg/mL,阿卡波糖的IC50为0.026 mg/mL,且其对α-葡萄糖苷酶的抑制作用表现为竞争性、可逆抑制,同时0.08 mg/mL浓度的柚皮苷具有较好的DPPH自由基清除能力。基于化橘红柚皮苷具有一定的抗氧化活性及对α-葡萄糖苷酶具有较强的抑制作用,可将其进一步用于天然抗氧化剂和α-葡萄糖苷酶抑制剂的开发。Theα-glucosidase inhibition activities of naringin from Exocarpium citri Grandis were investigated in order to provide reference for future exploitation of Exocarpium citri Grandis functional products.The scavenging ability and inhibition rate ofα-glucosidase of naringin were determined by 1,1-diphenyl-2-picryl-2-picrylhydrazyl(DPPH)radical scavenging and in vitroα-glucosidase inhibition model.Taking the inverse of the substrate concentration as the horizontal coordinate,the inhibition type ofα-glucosamine of samples was analyzed by using the inverse of the reflection rate as the longitudinal coordinate graph combined kinetic experiment.The inhibition activities of naringin from Exocarpium citri Grandis were higher than that of acarbose.The naringin could inhibit the activity ofα-glucosidase in a dose-and time-dependent manner.The half-inhibitory concentration(IC50)ofα-glucosidase was 0.008 mg/mL,while the IC50 of acarbose was 0.026 mg/mL.The inhibition ofα-glucosidase was reversible inhibition,and its action was competitive inhibition.Therefore,the naringin from Exocarpium citri Grandis was a good source of naturalα-glucosidase inhibitor and antioxidants,due to its certain antioxidant activity and its strong inhibition onα-glucosidase and.

关 键 词：化橘红 柚皮苷 Α-葡萄糖苷酶 抑制能力 

分 类 号：R285[医药卫生—中药学]