Single-cell RNA sequencing identifies novel cell types in Drosophila blood  

作　　者：Yulong Fu Xiaohu Huang Peng Zhang Joyce van de Leemput Zhe Han 

机构地区：[1]Center for Precision Disease Modeling,Department of Medicine,University of Maryland School of Medicine,Baltimore,MD,21201,USA [2]Divisions of Immunotherapy,University of Maryland School of Medicine,Baltimore,MD,21201,USA [3]Division of Endocrinology,Diabetes and Nutrition,Department of Medicine,University of Maryland School of Medicine,Baltimore,MD,21201,USA

出　　处：《Journal of Genetics and Genomics》2020年第4期175-186,共12页遗传学报（英文版）

基　　金：supported by grants from NIH to Z.H.(R01-HL134940,R01-DK098410)。

摘　　要：Drosophila has been extensively used to model the human blood-immune system,as both systems share many developmental and immune response mechanisms.However,while many human blood cell types have been identified,only three were found in flies:plasmatocytes,crystal cells and lamellocytes.To better understand the complexity of fly blood system,we used single-cell RNA sequencing technology to generate co mprehensive gene expression profiles for Drosophila circulating blood cells.In addition to the known cell types,we identified two new Drosophila blood cell types:thanacytes and primocytes.Thanacytes,which express many stimulus response genes,are involved in distinct responses to different types of bacteria.Primocytes,which express cell fate commitment and signaling genes,appear to be involved in keeping stem cells in the circulating blood.Furthermore,our data revealed four novel plasmatocyte subtypes(Ppn+,CAH7^+,Lsp^+and reservoir plasmatocytes),each with unique molecular identities and distinct predicted functions.We also identified cross-species markers from Drosophila hemocytes to human blood cells.Our analysis unveiled a more complex Drosophila blood system and broadened the scope of using Drosophila to model human blood system in development and disease.

关 键 词：DROSOPHILA BLOOD Single-cell RNA-seq Thanacyte Primocyte PLASMATOCYTE 

分 类 号：R346[医药卫生—基础医学]