离子液体辅助双水相系统提取茶渣中茶多酚工艺优化  

Optimization of the Extraction Technology of Tea Polyphenols from Tea Waste by Aqueous Two-phase Systems with Ionic Liquids as Additives

作　　者：龚新怀 李明春[2] 辛梅华[2] 赵晓杰[1] 吕橄 徐婕 赵升云 GONG Xin-huai;LI Ming-chun;XIN Mei-hua;ZHAO Xiao-jie;LV Gan;XU Jie;ZHAO Sheng-yun(Ecology and Resources Engineering College,Wuyi University,Fujian Provincial Key Laboratory of Eco-Industrial Green Technolog,Wuyislian 354300,China;College of Material Science and Engineering,Huaqiao University,Engineering Research Center of Environment-Friendly Functional Materials,Ministry of Education,Xiamen 361021,China)

机构地区：[1]武夷学院生态与资源工程学院,福建省生态产业绿色技术重点实验室,福建武夷山354300 [2]华侨大学材料科学与工程学院,环境友好功能材料教育部工程研究中心,福建厦门361021

出　　处：《食品工业科技》2020年第15期158-166,共9页Science and Technology of Food Industry

基　　金：福建省自然科学基金项目(2018J01445);福建省高校杰出青年科研人才计划(闽科教[2018]47号);福建省中青年教师教育科研项目(JAT170594,JT180555);武夷学院引进人才科研启动项目(YJ201810);武夷学院师生共创科研团队项目(2020-SSTD-009)。

摘　　要：为发展一种绿色、高效及温和的茶多酚提取方法,采用离子液体(IL)辅助乙醇/硫酸铵双水相系统(ATPS)提取茶渣中的茶多酚,考察了离子液体结构和用量对茶多酚得率和抗氧化性的影响,对提取工艺进行了正交试验优化。结果显示,少量IL添加可提高乙醇/硫酸铵ATPS的分相能力及其对茶多酚的得率,但对提取液的抗氧化活性没有明显影响;咪唑型离子液体对提取茶多酚具有更好的促进作用,咪唑环上侧链越长,其茶多酚得率越高;[C4mim]Cl辅助乙醇/硫酸铵ATPS对茶渣中茶多酚得率明显高于其他体系,且其提取液中儿茶素类组分含量更高。茶渣多酚最佳提取工艺为[C4mim]Cl质量分数10%、硫酸铵质量分数30%、乙醇体积分数60%,料液比1∶40 g/m L及超声功率540 W,在此条件下,茶渣中茶多酚得率为85.31±1.25 mg·g^-1。IL辅助双水相系统是一种有前景的茶多酚提取工艺,在保持茶多酚活性的同时,能获得较高的茶多酚得率和儿茶素类组分含量。In order to propose a green,efficient and gentle method for extraction of tea polyphenols,ionic liquids(ILs)assisted ethanol/ammonium sulphate aqueous two phase system(C2 H5 OH/(NH4)2 SO4 ATPS)was used as a alternative technique for extraction of the tea polyphenols in tea biomass,the effects of ionic liquids structures and amounts on the yield and antioxidant activity of tea polyphenols were studied,the optimal extraction was explored by orthogonal test.The results showed that the C2 H5 OH/(NH4)2 SO4 ATPS added with ILs as adjuvants in small amounts displayed a better phase separation and a higher yield,but no notable influence on the antioxidant activity of the extracts.The ILs with imidazolium cation showed a better improvement in extraction of tea polyphenols,and higher yield of tea polyphenols was obtained when the imidazolium ionic liquids with a longer side chain.The[C4 mim]Cl assisted C2 H5 OH/(NH4)2 SO4 ATPS showed a higher yield of polyphenols and a higher content of catechin than other extraction systems.The optimal extraction conditions of tea polyphenols concluded by the corresponding surface optimization method was that:[C4 mim]Cl and(NH4)2 SO4 mass fraction was 10%and 30%,ethanol volume fraction was 60%,liquid to solid ratio was 1∶40 g/m L,ultrasonic power was 540 W,thus the yield of tea polyphenols obtained was 85.31±1.25 mg·g^-1 at the conditions.Ionic liquids assisted ATPS showed an promising extraction of polyphenols,while the biological activities of the tea polyphenols were remained and a higher yield of polyphenols and content of catechin components were obtained.

关 键 词：茶多酚 双水相系统 离子液体 得率 抗氧化性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]