乳酸链球菌素与L-乳酸对嗜水气单胞菌的协同抑杀作用  

Synergistic Inhibitory Effects of Nisin and L-Lactic Acid against Aeromonas hydrophila

作　　者：王洋 王竞儒 白东清 淡清华 于波 余政豪 罗云龙 WANG Yang;WANG Jing-ru;BAI Dong-qing;DAN Qing-hua;YU Bo;YU Zheng-hao;LUO Yun-long(Tianjin Key Laboratory of Aqua-ecology and Aquaculture,College of Fisheries,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384

出　　处：《食品工业科技》2020年第16期81-87,共7页Science and Technology of Food Industry

基　　金：国家自然科学基金(31702360);国家级大学生创新创业训练计划项目(201910061016);天津市水产产业技术体系创新团队(ITTFRS2017003,ITTFRS2017004,ITTFRS2018048)。

摘　　要：为探讨乳酸链球菌素(Nisin)与L-乳酸对嗜水气单胞菌的协同抑杀作用,分别采用比浊法、活菌计数法、膜电动势探针等检测Nisin与L-乳酸对嗜水气单胞菌(ATCC 35654、CICC 10500两株菌)生长、存活、生物被膜形成以及菌体膜完整性的影响。结果表明,Nisin与低浓度(3.5 mmol/L)L-乳酸协同,显著抑制了两株嗜水气单胞菌的生长,使生长延滞期延长,最大比生长速率下降,成熟期最高活菌数量降低,且抑制效果随Nisin浓度增加而增强。Nisin和L-乳酸对指示菌有协同致死作用,Nisin与L-乳酸协同处理12 h,可显著(P<0.05)降低嗜水气单胞菌活菌数,造成菌体细胞死亡。机理初探表明:低浓度L-乳酸可以使菌株外膜脂多糖(Lipopolysaccharide,LPS)释放,Nisin进一步使菌体膜电动势消散。研究结果为利用Nisin与L-乳酸或二者的产生菌控制水产品中的嗜水气单胞菌提供了理论依据。To investigate the synergistic inhibitory effect and mechanism of nisin and L-lactic acid against Aeromonas hydrophila,turbidity,plate count method,membrane electromotive force probe were used to detect the influence of nisin and L-lactic acid on growth,survival,biofilm formation,and membrane integrity of Aeromonas hydrophila(ATCC 35654,CICC 10500). The results showed that nisin and low concentration(3.5 mmol/L)of L-lactic acid synergistically inhibited the growth of two strains of Aeromonas hydrophila. And the inhibitory effect strongthened with the increasing of nisin concentration. The growth parameters modeled with a Gomptz equation showed that nisin and L-lactic acid synergistically prolonged the growth lag phase of Aeromonas hydrophila,the maximum specific growth rate decreased,and the maximum viable count of bacteria at stationary phase also decreased. Besides,nisin and L-lactic acid had a synergistic lethal effect on the indicator bacteria. Nisin and L-lactic acid treated for 12 h reduced the viable count of Aeromonas hydrophila. Results of the mechanism indicated that low concentration of L-lactic acid released the outer membrane LPS from the bacterial cells,and nisin further dissipated the membrane potential. The results would provide a theoretical basis for the use of nisin and L-lactic acid or their producing strains in control of Aeromonas hydrophila in aquatic products.

关 键 词：L-乳酸 乳酸链球菌素 嗜水气单胞菌 生长 存活 生物被膜 膜完整性 

分 类 号：TS201.3[轻工技术与工程—食品科学]