超声提取柿果皮总三萜酸工艺优化  

Optimization of ultrasonic extraction technology of total triterpenoid acid from persimmon peel

作　　者：施佳男 曹超 许佳玉 周春华[1] SHI Jia-Nan;CAO Chao;XU Jia-Yu;ZHOU Chun-Hua(College of Horticulture and Plant Protection,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学园艺与植物保护学院,扬州225009

出　　处：《食品安全质量检测学报》2020年第14期4574-4580,共7页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31372042);扬州大学优秀青年骨干教师项目(2014)。

摘　　要：目的采用正交法优化柿果皮中总三萜酸的超声提取工艺。方法以"牛心柿"柿果皮为原料,利用紫外分光光度计法测定柿果皮中总三萜酸的含量,并从提取溶剂选择及其浓度、料液比、超声时间和温度等方面,通过单因素实验和正交试验确定超声提取柿果皮总三萜酸的最佳条件。结果最佳超声提取工艺为:在超声功率240 W的前提下,提取溶剂为75%(V/V)的乙醇、料液比1:15(m/V)、时间45 min、温度50℃。在此条件下,柿果皮总三萜得率最高可达39.33mg/g。结论优化后的方法简单、快速、准确、提取率高,适合提取柿果皮的总三萜酸。Objective To optimize the ultrasonic extraction technology of total triterpenoid acid in persimmon peel by orthogonal design.Methods With the peel of"Niuxinshi"as raw material,the total triterpenoid acid in persimmon peel was determined by spectrophotometer,and the best conditions of extracting total triterpenoid acid from persimmon peel was determined by single factor experiment and orthogonal experiment from the aspects of solvent selection and concentration,material liquid ratio,ultrasonic time and temperature.Results The optimal ultrasonic extraction conditions was as follow:under the condition of 240 W ultrasonic power,75%(V/V)ethanol as the extraction solvent,the solid-liquid ratio 1:15(m/V),the extraction time 45 min and the temperature 50℃.Under this condition,the total triterpenoid acid yield in persimmon peel was up to 39.33 mg/g.Conclusion The optimized method is simple,rapid,accurate and suitable for the extraction of total triterpenoid acid from persimmon peel.

关 键 词：柿果皮 总三萜酸 超声提取 正交法 

分 类 号：O657.32[理学—分析化学] TS201.2[理学—化学]