婴儿配方乳粉中4种植物甾醇的含量研究  

Determination the content of four phytosterols in infant formula milk powder

作　　者：符昌雨[1] 杜茹芸 麦成华[1] FU Chang-Yu;DU Ru-Yun;MAI Cheng-Hua(Shanghai Institute of Quality Inspection and Technical Research/National Food Quality Supervision and Inspection Center,Shanghai 200233,China)

机构地区：[1]上海市质量监督检验技术研究院/国家食品质量监督检验中心,上海200233

出　　处：《食品安全质量检测学报》2020年第14期4649-4652,共4页Journal of Food Safety and Quality

摘　　要：目的气相色谱法分析我国市场上销售的30批次婴儿配方乳粉,研究其中的4种植物甾醇含量分布情况。方法样品中加入内标5-α胆甾烷醇溶液和10%乙酰氯甲醇溶液,在80℃水浴中甲酯化2 h,用甲苯萃取。加入碳酸钠溶液(6 g/100 mL)使其分层,取上层甲苯溶液氮吹浓缩后,定容至1.0 mL,用气相色谱仪分析。结果婴儿配方乳粉中4种植物甾醇含量在23.9~148.9 mg/kg之间,β-谷甾醇含量最高,分别占到各自乳粉中4种甾醇总量的40.9%~69.0%。结论此次检测的30批次婴儿配方乳粉中均含有一定量的植物甾醇,但样品中的甾醇含量不同。Objective To analyze 30 batches of infant formula milk powder sold in China’s market by gas chromatography,the content distribution of 4 phytosterols was studied.Methods The internal standard 5-cholesterol solution and 10%acetyl chloromethanol solution were added to the sample,then methyl esterified in 80℃water bath for 2 h and extracted with toluene.Sodium carbonate solution(6 g/100 mL)was added to stratify it,and the upper toluene solution was concentrated by nitrogen blowing,and the volume was constant to 1.0 mL,which was analyzed by gas chromatography.Results The content of 4 phytosterols in infant formula milk powder was between 23.9-148.9 mg/kg,and the content ofβ-sitosterol was the highest,accounting for 40.9%-69.0%of the total amount of four sterols in each milk powder.Conclusion The 30 batches of infant formula milk powder tested this time all contain a certain amount of phytosterol,but the sterol content in the samples is different.

关 键 词：植物甾醇 气相色谱法 婴儿配方乳粉 测定 

分 类 号：TS252.51[轻工技术与工程—农产品加工及贮藏工程] O657.71[轻工技术与工程—食品科学与工程]