‘塔罗科’血橙成熟过程中花色苷积累及其与糖酸含量相关性  

Correlation between Anthocyanin Accumulation and Sugar and Acid Contents in‘Tarocco’Blood Oranges during Ripening

作　　者：喻最新 王日葵[1] 贺明阳[1] 洪敏 袁小淞 王晶 冯雨 吴志刚 YU Zuixin;WANG Rikui;HE Mingyang;HONG Min;YUAN Xiaosong;WANG Jing;FENG Yu;WU Zhigang(Citrus Research Institute,Southwest University,Chongqing 400712,China)

机构地区：[1]西南大学柑桔研究所,重庆400712

出　　处：《食品科学》2020年第15期105-114,共10页Food Science

基　　金：重庆市基础研究与前沿探索项目(cstc2018jcyj AX0256);中央高校基本科研业务费专项资金项目(XDJK2017C016);重庆市科技专项(cstc2016shms-ztzx80005);中国博士后科学基金项目(2018M633178)。

摘　　要：本实验通过测定和分析‘塔罗科’血橙成熟过程中(开花后200~324 d)花色苷质量浓度、可溶性糖含量、有机酸含量、花色苷合成相关基因表达量的动态变化,研究花色苷积累及其与糖酸含量的相关性。结果表明:在‘塔罗科’血橙成熟过程中,果实中的花色苷质量浓度呈逐渐上升的趋势。开花后261 d开始在血橙汁中检测到花色苷,开花后276~293 d是血橙果实花色苷的快速积累时期,在此期间血橙汁中的花色苷质量浓度日均提高0.55 mg/L。4CL、CHS、F3H、F3’5’H、DFR、ANS、UFGT、GST基因是调控血橙果实花色苷积累的核心结构基因,Ruby是调控血橙果实花色苷合成的关键转录因子。血橙成熟过程中花色苷质量浓度与果糖、葡萄糖含量呈极显著正相关(P<0.01),相关系数分别为0.810和0.799。成熟过程中血橙果实中果糖、葡萄糖的积累可能是促进花色苷合成的重要原因。In the present study, the variation in anthocyanin content, soluble sugar, organic acid content and anthocyanin synthesis-related gene expression in Tarocco blood oranges during the ripening period(from Day 200 to Day 324 after flowering) were investigated to study the correlation between anthocyanin accumulation and soluble sugar and organic acid contents. Results showed that anthocyanin content in blood oranges gradually increased during the ripening period. The presence of anthocyanins was detected for the first time in blood orange juice at 261 days after flowering. Rapid accumulation of anthocyanins in blood oranges occurred from Day 276 to Day 293 after flowering, with an increasing rate of 0.55 mg/L per day during this period. 4 CL, CHS, F3 H, F3’5’H, DFR, ANS, UFGT and GST were the core structural genes that regulate the accumulation of anthocyanins in blood oranges. Ruby was the key transcription factor regulating the biosynthesis of anthocyanins in blood oranges. The positive correlation of anthocyanin content with fructose and glucose contents was extremely significant(P < 0.01), with correlation coefficients of 0.810 and 0.799, respectively. The accumulation of fructose and glucose in blood oranges during the ripening period may be an important reason for the biosynthesis of anthocyanins.

关 键 词：血橙 成熟 花色苷 可溶性糖 有机酸 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]