响应面法优化微波辅助酶解制备抗性淀粉工艺研究  

Optimization of Microwave-assisted Enzymatic Hydrolysis for the Preparation of Resistant Starch by Response Surface Methodology

作　　者：张守花[1] ZHANG Shou-hua(Hebi Polytechnic,Hebi 458030,Henan,China)

机构地区：[1]鹤壁职业技术学院,河南鹤壁458030

出　　处：《食品研究与开发》2020年第17期122-127,共6页Food Research and Development

基　　金：河南省高等学校重点科研项目计划(19B550004)。

摘　　要：采用微波辅助酶解制备玉米抗性淀粉,以玉米抗性淀粉收率为指标,在单因素试验基础上,进行Box-Behnken试验设计,对耐高温α-淀粉酶添加量和酶解时间、普鲁兰酶添加量和酶解时间4个因素进行响应面优化试验分析。结果表明4个因素的影响主次关系为普鲁兰酶酶解时间>耐高温α-淀粉酶酶解时间>耐高温α-淀粉酶添加量>普鲁兰酶添加量。响应面优化试验确定微波辅助酶解制备玉米抗性淀粉的最优工艺参数:耐高温α-淀粉酶添加量3 U/g干淀粉、酶解时间30 min,普鲁兰酶添加量8 U/g干淀粉、酶解时间4.5 h。Corn resistant starch was prepared by microwave-assisted enzymatic hydrolysis.Based on the yield of corn resistant starch,Box-Behnken test design was carried out on the basis of single factor test.Response surface optimization test analysis was carried out on four factors of high temperature resistanceα-amylase addition amount and enzymolysis time,pullulanase addition amount and enzymolysis time.The results showed that the primary and secondary relationship of the four factors was the time of enzymatic hydrolysis of pullulanase,the time of enzymatic hydrolysis of high temperature resistantα-amylase,the amount of high temperature resistantα-amylase and the amount of pullulanase.Response surface optimization test determined the optimal processing parameters of microwave-assisted enzymatic hydrolysis of corn resistant starch:high temperature resistantα-amylase 3 U/g dry starch,enzymatic hydrolysis time 30 min,pullulanase 8 U/g dry starch,enzymatic hydrolysis time 4.5 h.

关 键 词：响应面法 微波辅助酶解 玉米抗性淀粉 耐高温Α-淀粉酶 普鲁兰酶 

分 类 号：TS234[轻工技术与工程—粮食、油脂及植物蛋白工程]