马氏珠母贝黏液糖蛋白分离纯化及其抗氧化活性研究  

Study on extraction and purification of glycoprotein from Pinctada martensii’ mucus and its antioxidant activity

作　　者：余蔚 欧阳彤 罗碧琦 伍彬[1] 郑惠娜[1,2,3] 曹文红 林海生[1,2,3] 章超桦 秦小明[1,2,3] YU Wei;OUYANG Tong;LUO Biqi;WU Bin;ZHENG Huina;CAO Wenhong;LIN Haisheng;ZHANG Chaohua;QIN Xiaoming(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Shenzhen Institute of Guangdong Ocean University,Shenzhen 518108,China;National Research and Development Branch Center for Shellfish Processing(Zhanjiang)/Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety/Guangdong Province Engineering Laboratory for Marine Biological Products,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东海洋大学深圳研究院,广东深圳518108 [3]国家贝类加工技术研发分中心(湛江)/广东省水产品加工与安全重点实验室/广东省海洋生物制品工程实验室,广东湛江524088

出　　处：《南方水产科学》2020年第4期100-107,共8页South China Fisheries Science

基　　金：广东省自然科学基金项目(2016A030313810);广东省现代农业产业技术体系创新团队项目(2019KJ146);广东省科技计划项目(2013B020312006);国家贝类产业技术体系项目(CARS-49);广东海洋大学大学生创新创业训练计划项目(CXXL2019155)。

摘　　要：该研究采用直接醇沉法、盐提醇沉法和水提醇沉法提取马氏珠母贝(Pinctada martensii)黏液中的糖蛋白,优选直接醇沉法粗品(DAS),进一步采用Sephacryl S-200纯化获得直接醇沉纯化品(DAPS),同时测定DAS及DAPS的体外抗氧化活性。结果显示,DAS的糖和蛋白质得率均最高,且3种方法提取的糖蛋白粗品蛋白质组成无明显差异;DAPS的总糖和蛋白质质量分数分别为(22.72±0.26)%和(71.18±0.62)%,分子量主要集中在245kD;DAS和DAPS对羟自由基的清除能力显著,在0.8 mg·mL^-1下两者对羟自由基的清除率均高于90%,对超氧阴离子自由基及DPPH自由基也有一定清除能力。研究结果可为探索马氏珠母贝黏液功效组分提供理论基础数据。In this study,we used direct alcohol precipitation,salt extraction and alcohol precipitation,water extraction and alcohol precipitation to extract the glycoprotein from Pinctada martensii’mucus,and the direct alcohol precipitation sample(DAS)was preferred.We obtained the direct alcohol precipitation and purification sample(DAPS)by Sephacryl S-200 and determined antioxidant activity of DAS and DAPS in vitro.The results show that DAS had the highest yields of sugar and protein,and there is no significant difference in the protein composition of the crude glycoproteins extracted by the three methods.The total sugar and protein contents of DAPS were(22.72±0.26)%and(71.18±0.62)%,respectively,and the molecular weight was mainly 245 kD.Both DAS and DAPS had certain antioxidant activity.The scavenging abilities of DAS and DAPS to hydroxyl radical were significant,and their scavenging rates to hydroxyl radical were higher than 90%at 0.8 mg·mL-1.Both had scavenging activity to superoxide anion free radical and DPPH free radical.The results provide theoretical basis data for exploring the active components of P.martensii’mucus.

关 键 词：马氏珠母贝黏液 糖蛋白 抗氧化活性 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]