一株整合型高抗性高效发酵己酸菌的选育研究  

Breeding of an integrated strain with high resistance and highly efficient fermentation for caproic acid production

作　　者：薛正楷[1,2] 郑文武 张宿义 XUE Zhengkai;ZHENG Wenwu;ZHANG Suyi(School of Liquor-making Engineering,Luzhou Vocational and Technical College,Luzhou 646001,China;Luzhou Institute of Biomedical Engineering,Luzhou 64600,China;The Affiliated Hospital of Southwest Medical University,Luzhou 646001,China;Luzhou Laojiao Group Co.,Ltd.,Luzhou 646002,China)

机构地区：[1]泸州职业技术学院白酒学院,四川泸州646001 [2]泸州市生物医学工程研究所,四川泸州646000 [3]西南医科大学附属医院,四川泸州646001 [4]泸州老窖股份有限责任公司,四川泸州646002

出　　处：《中国酿造》2020年第8期143-150,共8页China Brewing

基　　金：四川省科技支撑计划项目(NO.2014FZ0018);四川省教育厅理工重点项目(18ZA0250);泸州市科学技术和知识产权局社会发展项目(2017-S-45(3/3))。

摘　　要：该试验采用同源重组技术从产己酸菌-纺锤形赖氨酸芽孢杆菌(Lysinibacillus fusiformis)基因组中敲出α-淀粉酶基因、蔗糖磷酸化酶基因,同时敲进SigA和SigB基因,以提高L.fusifoemis的抗性,选育高效发酵己酸菌。结果表明,SigA和SigB双整合菌株SigA-SigB4在pH 4、乙醇体积分数6%条件下,己酸产量达到360.86 mg/100 mL,比单整合菌株SigB3提高了1.17倍,比非重组出发株L.fusifoemis提高了8.97倍,且经过32代传代后,其高效产己酸效能无显著变化(P>0.05)。In this study,theα-amylase gene and sucrose phosphorylase gene from the genome of caproic acid-producing Lysinibacillus fusiformis were knocked out by homologous recombination technology,and at the same time the SigA and SigB genes were knocked in to improve the resistance of L.fusifoemis,and then select and breed caproic acid bacteria with highly efficient fermentation.The results showed that the yield of caproic acid reached 360.86 mg/100 ml,which was 1.17 times higher than that of integration strain SigB3,and 8.97 times higher than that of the non-recombinant strain L.fusifoemis under the conditions of pH 4 and ethanol volume fraction 6%of integration strains SigA-SigB4 with SigA and SigB genes.Moreover,the production efficiency of caproic acid did not change significantly(P>0.05)after 32 generations.

关 键 词：整合型 产己酸菌 选育 发酵 

分 类 号：TS261.1[轻工技术与工程—发酵工程]