Brain interstitial fluid drainage and extracellular space affected by inhalational isoflurane: in comparison with intravenous sedative dexmedetomidine and pentobarbital sodium  

作　　者：Guomei Zhao Hongbin Han Jun Yang Min Sun Dehua Cui Yuanyuan Li Yajuan Gao Jing Zou 

机构地区：[1]Department of Radiology,Peking University Third Hospital,Beijing 100191,China [2]Beijing Key Laboratory of Magnetic Resonance Imaging Technology,Beijing 100191,China [3]Institute of Medical Technology,Peking University,Beijing 100191,China [4]Department of Neurosurgery,Peking University Third Hospital,Beijing 100191,China [5]Department of Cardiology,Peking University Shougang Hospital,Beijing 100144,China

出　　处：《Science China(Life Sciences)》2020年第9期1363-1379,共17页中国科学（生命科学英文版）

基　　金：supported by the National Major Scientific Research Instrument Development Project(61827808);the National Key Basic Research Program(973 Program,2015CB856402);Beijing Brain Initiative of Beijing Municipal Science&Technology Commission(Z181100001518004);National Natural Science Foundation of China(81872051);Peking University Clinical Scientist Program(BMU2019LCKXJ007)。

摘　　要：Brain interstitial fluid drainage and extracellular space are closely related to waste clearance from the brain. Different anesthetics may cause different changes of brain interstitial fluid drainage and extracellular space but these still remain unknown. Herein,effects of the inhalational isoflurane, intravenous sedative dexmedetomidine and pentobarbital sodium on deep brain matters’ interstitial fluid drainage and extracellular space and underlying mechanisms were investigated. When compared to intravenous anesthetic dexmedetomidine or pentobarbital sodium, inhalational isoflurane induced a restricted diffusion of extracellular space, a decreased extracellular space volume fraction, and an increased norepinephrine level in the caudate nucleus or thalamus with the slowdown of brain interstitial fluid drainage. A local administration of norepinephrine receptor antagonists, propranolol,atipamezole and prazosin into extracellular space increased diffusion of extracellular space and interstitial fluid drainage whilst norepinephrine decreased diffusion of extracellular space and interstitial fluid drainage. These findings suggested that restricted diffusion in brain extracellular space can cause slowdown of interstitial fluid drainage, which may contribute to the neurotoxicity following the waste accumulation in extracellular space under inhaled anesthesia per se.

关 键 词：interstitial fluid deep brain extracellular space ISOFLURANE DEXMEDETOMIDINE pentobarbital sodium NOREPINEPHRINE 

分 类 号：R614[医药卫生—麻醉学]