基于PacBio平台的紫娟茶树全长转录组分析  

Full-Length Transcriptome Analysis of Zijuan Tea(Camellia sinensis var. asssamica(Masters) kitamura) Base on PacBio Platform

作　　者：夏丽飞[1] 孙云南[1] 宋维希[1] 朱兴正[1] 田易萍[1] 蒋会兵[1] 陈林波[1] Xia Lifei;Sun Yunnan;Song Weixi;Zhu Xingzheng;Tian Yiping;Jiang Huibin;Chen Linbo(Tea Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Technology Engineering Research Center of Tea Germplasm Innovation and Supporting Cultivation,Yunnan Provincial Key Laboratory of Tea Science,Menghai,666201)

机构地区：[1]云南省农业科学院茶叶研究所,云南省茶树种质资源创新与配套栽培技术工程研究中心,云南省茶学重点实验室,勐海666201

出　　处：《基因组学与应用生物学》2020年第6期2646-2658,共13页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31560220);茶树生物学与资源利用国家重点实验室开放基金(SKLTOF20150105);云南省人才培养计划项目(2015HB105)共同资助。

摘　　要：紫娟(Camellia sinensis var. Asssamica (Masters) kitamura)是珍稀茶树种质的典型代表,其叶片色泽呈现紫色,富含花青素、儿茶素以及黄酮等活性成分。为探讨紫娟茶树叶片呈色以及次级代谢过程的遗传基础,以紫娟茶树为材料,采用Pac Bio平台进行全长转录组测序,最终获得Polished consensus序列205 911个。在NR数据库有163 519个同源序列比对到914个物种;有99 503个在KOG数据库得到注释,根据其功能分为26类;有76 829个得到GO注释,分为细胞组分、分子功能及生物学过程等3大类的54个功能组;根据KEGG数据库,109 748个被注释到216个代谢途径分支,其中氨基酸代谢的7 731个、类黄酮生物合成的有644个、单萜的合成的有57个、萜类化合物骨架生物合成487个、黄酮和黄酮醇的生物合成的有88个、花青素合成1个;同时预测到CDS有199 245个、转录因子有6 838个;此外,还检测到了180 293个SSR位点,其中以单碱基重复最丰富有103 535个,其次是双碱基43 240和三碱基30 883。本研究结果为开展紫娟茶树叶片呈色机理以及花青素、黄酮类等物质合成途径与调控机制和特异性状基因的标记性引物开发提供重要的数据支持。Zijuan(Camellia sinensis var. asssamica(Masters) kitamura), a typical representative of rare tea plants.The bud, leaf, and stem of the fresh Zijuan tea shoot appear purple. They are rich in various bio-active substances,such as anthocyanin, catechin, flavoniod, etc. In order to explore genetic basis for research coloration mechanism and the synthesis of the secondary metabolites, the full-length transcriptome of Zijuan tea plant was sequenced by PacBio platform. A total of 205 911 polished consensus were generated. 99 503 Unigenes were annotated against the Nr databases. According to KOG databases, 160 126 Unigenes were further annotated and grouped into 26 functional categories. 76 829 Unigenes were annotated against GO database were divided into cellular component,molecular function and biological process categories with a total of 54 functional groups. KEGG pathway analysis showed that 109 748 Unigenes could be broadly classified into 216 classes according to their function, and some of them were involved in amino acid metabolism(7 731), flavonoid biosynthesis(644), monoterpenoid biosynthesis(57), terpenoid backbone biosynthesis(487), flavone and flavonol biosynthesis(88) and anthocyanin biosynthesis(1). Additionally, 199 245 CDS and 6 838 transcription factor were predicted. In addition, 180 293 simple sequence repeats were detected. The most abundant microsatellites were mononucleotide repeats, with the SSR numbers being 103 535, followed by trinucleotide and double base repeats, being 43 240 and 30 883, respectively.The results of this study provides a useful resource for research coloration mechanism of zijuan tea plant, and studying synthesis pathwayand regulation mechanism of anthocyanins and flavonoids substances.

关 键 词：紫娟茶树 全长转录组 测序分析 功能注释 

分 类 号：S571.1[农业科学—茶叶生产加工]