超高效液相色谱-串联质谱法测定鸡肉、鸡蛋中氟苯尼考和氟苯尼考胺残留  

Determination of florfenicol and florfenicol amine residues in chicken and hen eggs by ultra performance liquid chromatography tandem mass spectrometry

作　　者：王丽英[1] 任贝贝[1] 路杨[1] 刘梦颖 刘印平[1] 冯静[1] 常凤启[1] WANG Li-Ying;REN Bei-Bei;LU Yang;LIU Meng-Ying;LIU Yin-Ping;FENG Jing;CHANG Feng-Qi(Hebei Provincial Center for Disease Control and Prevention,Shijiazhuang 050021,China)

机构地区：[1]河北省疾病预防控制中心,石家庄050021

出　　处：《食品安全质量检测学报》2020年第15期5056-5061,共6页Journal of Food Safety and Quality

摘　　要：目的建立超高效液相色谱-串联质谱准确测定鸡肉、鸡蛋中氟苯尼考和氟苯尼考胺残留的分析方法。方法鸡肉和鸡蛋样品加入D5-氯霉素内标,经氨化乙酸乙酯混合溶液超声离心提取,提取液减压蒸馏浓缩后,C18固相萃取柱净化处理,采用超高效液相色谱-串联质谱法对氟苯尼考和氟苯尼考胺同时进行检测。结果氟苯尼考线性范围为0.2~20μg/L,检出限为1.0μg/kg,定量限为3.0μg/kg,方法回收率为88.0%~108.0%,相对标准偏差为4.7%~6.4%;氟苯尼考胺线性范围为0.2~20μg/L,检出限为1.0μg/kg,定量限为3.0μg/kg,方法回收率为76.0%~93.1%,相对标准偏差为4.1%~7.2%。结论本方法精确、重现性好,适用于鸡肉、鸡蛋中氟苯尼考和氟苯尼考胺残留量的测定。Objective To establish a method for the determination of florfenicol and florfenicol amine residues in chicken and hen eggs by ultra performance liquid chromatography-tandem mass spectrometry. Methods Chicken and hen eggs samples were added to the internal standard of D5-chloramphenicol and extracted by ultrasonic centrifugation with ammoniated ethyl acetate mixed solution. After the extract was concentrated under reduced pressure, purified by the C18 solid-phase extraction column, florfenicol and florfenicol amine residues were simultaneous determined by ultra performance liquid chromatography tandem mass spectrometry. Results The linear range of florfenicol was 0.2–20 μg/L, the detection limit was 1.0 μg/kg, the limit of quantification was 3.0 μg/kg, the method recoveries were 88.0%–108.0%, and the relative standard deviations were 4.7%-6.4%. The linear range of florfenicol amine was 0.2–20 μg/L, the detection limit was 1.0 μg/kg, the limit of quantification was 3.0 μg/kg, the method recoveries were 76.0%–93.1%, and the relative standard deviations were 4.1%–7.2%. Conclusion The method is accurate and reproducible, and is suitable for the determination of florfenicol and florfenicol amine residues in chicken and hen eggs.

关 键 词：氟苯尼考 氟苯尼考胺 鸡肉 鸡蛋 超高效液相色谱-串联质谱法 

分 类 号：O657.63[理学—分析化学] TS251.55[理学—化学]