铜绿假单胞菌M19降解黄曲霉毒素B1的产物研究  

Analysis of the Degradation Products of Aflatoxin B1 by Pseudomonas aeruginosa M19

作　　者：王佳兴 谢岩黎[1] 宋娟娟 马卫宾 孙淑敏[1] 李倩 WANG Jia-xing;XIE Yan-li;SONG Juan-juan;MA Wei-bin;SUN Shu-min;LI Qian(College of Food Sience and Technology,Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control,Henan University of Technology,Zhengzhou,Henan 450001,China)

机构地区：[1]河南工业大学粮油食品学院,河南省粮油食品安全检测与控制重点实验室,河南郑州450001

出　　处：《粮油食品科技》2020年第5期1-5,共5页Science and Technology of Cereals,Oils and Foods

基　　金：河南省高校科技创新团队计划资助(20IRTSTHN023);河南省重点研发与推广专项(192102310254)。

摘　　要：以实验室筛选保藏的黄曲霉毒素B1(Aflatoxin B1,AFB1)降解菌M19产生的降解酶PADE为对象,对其AFB1降解液进行薄层色谱及荧光光谱分析(LC-MS),分析降解产物可能的结构变化,并利用液相质谱检测AFB1降解产物。薄层色谱检测结果:有机相和水相降解液均未检测到新的荧光吸收物质,表明降解产物没有荧光吸收;荧光光谱检测结果:AFB1降解产物的荧光明显减弱;液相质谱检测结果:发现质荷比为227.18的物质P。结果推断:AFB1降解过程中内酯键断裂,产生一个分子量为226的降解产物P,利用Xcalibur软件分析其分子式为C14H10O3,并根据AFB1降解后的LC-MS图谱分析以及AFB1降解产物的相关文献对降解途径进行假设。A degrading enzyme PADE produced during the degradation of aflatoxin B1(AFB1)by the laboratory screening bacteria M19 was selected as the subject in this study.Thin layer chromatography and fluorescence spectrum analysis were performed to analyze possible structural changes of products in the AFB1 degradation solution.Mass spectrometric were further used to detect the AFB1 degradation products.The results of thin layer chromatography showed that no new fluorescent absorbent substance was detected in the organic phase degradation solution,and no absorbent substance was detected in the aqueous phase degradation solution,indicating that the degradation product did not have fluorescence absorption.The fluorescence spectrum detection results showed that the fluorescence of the AFB1 degradation product was decreased obviously.The mass spectrometry results showed that a substance P with a mass-to-charge ratio of 227.18 was found.Based on all the results,it can be concluded that the lactone bond was broken during the degradation of AFB1,and a degradation product P with a molecular weight of 226 was generated.The molecular formula was C14H10O3 as analyzed by Xcalibur software.The degradation pathway was hypothesized.

关 键 词：黄曲霉毒素B1 降解酶 降解产物 Xcalibur软件 荧光吸收物质 

分 类 号：TS201.6[轻工技术与工程—食品科学]