植物乳杆菌M1-NTG300产细菌素培养条件优化  

Optimizing conditions of producing bacteriocin by Lactobacillus plantarum M1-NTG300

作　　者：安宇[1] 王颖[1,2] 易华西 张东杰[1] AN Yu;WANG Ying;YI Hua-xi;ZHANG Dong-jie(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China;National Coarse Cereals Engineering Research Center,Daqing,Heilongjiang 163319,China;College of Food Science and Engineering,Ocean University of China,Qingdao,Shandong 266000,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]国家杂粮工程技术研究中心,黑龙江大庆163319 [3]中国海洋大学食品科学与工程学院,山东青岛266000

出　　处：《食品与机械》2020年第8期178-182,209,共6页Food and Machinery

基　　金：国家重点研发计划项目(编号:2018YFE0206300);国家“十二五”支撑计划项目(编号:2015BAD16B05)。

摘　　要：以植物乳杆菌素的效价作为特征性考察指标,对植物乳杆菌M1-NTG300产细菌素培养条件进行优化。通过响应面分析结合实际值确定培养基最佳组分为:葡萄糖浓度3.5%、蛋白胨浓度3.5%、吐温80浓度0.35%、氯化镁浓度0.20%,此培养条件下植物乳杆菌M1-NTG300所产细菌素的效价为1269.4 IU/mL,细菌素效价提高了19.8%。Taking the potency of phytolactin as a characteristic index,the culture conditions for bacteriocin production by Lactobacillus plantarum M1-NTG300 were optimized.Through response surface analysis combined with actual values,the optimal components of the culture medium were determined to include 3.5%glucose concentration,3.5%peptone,0.35%Tween 80,and 0.20%magnesium chloride.Under the control of these culture conditions,The titer of bacteriocin,produced by L.plantarum M1-NTG300 was 1269.4 IU/mL,which was increased by 19.8%.

关 键 词：植物乳杆菌 细菌素 培养基 

分 类 号：TS201.3[轻工技术与工程—食品科学]