沙门氏菌、金黄色葡萄球菌、志贺氏菌和单增李斯特菌选择性共增菌培养基的研制  

Preparation of Selective Co-enrichment Medium for Salmonella,Staphylococcus aureus,Shigella and Listeria monocytogenes

作　　者：严礼 肖稳 郭焜鹏 何娟 刘卓灵 余正 YAN Li;XIAO Wen;GUO Kun-peng;HE Juan;LIU Zhuo-ling;YU Zheng(Hunan Institute of Food Quality Supervision and Inspection,Changsha 410117,China;Cooperative Innovation Center of Jiangnan University,Wuxi 214100,China)

机构地区：[1]湖南省食品质量监督检验研究院(生物安全检验研究所),长沙410117 [2]江南大学(协同创新中心),江苏无锡214100

出　　处：《中国食物与营养》2020年第8期14-18,共5页Food and Nutrition in China

基　　金：湖南省食品药品安全科技项目(项目编号:湘食药科R201812)。

摘　　要：目的:研制一种对沙门氏菌(Salmonella)、金黄色葡萄球菌(Staphylococcus aureus)、志贺氏菌(Shigella)和单增李斯特菌(Listeria monocytogens)的选择性共增菌培养基(SSSL培养基)。方法:挑选添加成分进行单因素试验,确定SSSL培养基的成分及配比,采用平板计数法验证SSSL培养基的增菌效果。结果:确立了SSSL培养基配方,目标菌在SSSL增菌培养基中培养8 h后,菌体浓度都达到了105~106CFU/mL,而且抑制非目标菌的生长。结论:SSSL培养基能用于沙门氏菌、金黄色葡萄球菌、志贺氏菌和单增李斯特菌选择性共增菌,可望与多种检测方法联用,以提高检测率和准确性。Objective To develop a selective co-culture medium(SSSL)for Salmonella,Staphylococcus aureus,Shigella and Listeria monocytogens.Method Single factor experiments were conducted to determine the composition and proportion of SSSL medium,and plate counting method was used to verify the bacteriostasis effect of SSSL medium.Result The SSSL medium formulation was established.After 8 hours of culture in SSSL medium,the concentration of target bacteria reached 105~106 CFU/mL,and the growth of non-target bacteria was inhibited.Conclusion SSSL medium can be used for selective co-growth of Salmonella,Staphylococcus aureus,Shigella and Listeria monocytogenes.It is expected that SSSL medium can be combined with various detection methods to improve the detection rate and accuracy.

关 键 词：沙门氏菌 金黄色葡萄球菌 志贺氏菌 单增李斯特菌 共增菌 选择性培养基 

分 类 号：TS207.4[轻工技术与工程—食品科学]