不同地区大西洋鲑鱼无机元素含量的对比分析  

Comparison on Inorganic Elements Contents in Atlantic Salmon from Different Regions

作　　者：宋伟 沈贵兰 韩芳 邓晓军 周典兵 何雨婷 丁磊 吕亚宁 郑平 SONG Wei;SHEN Gui-lan;HAN Fang;DENG Xiao-jun;ZHOU Dian-bing;HE Yu-ting;DING Lei;LV Ya-ning;ZHENG Ping(Technical Center for Hefei Customs,Hefei 230022,China;Anhui Province Key Laboratory of Analysis and Detection for Food Safety,Hefei 230022,China;Shanghai Academy of Science and Technology for Inspection and Quarantine,Shanghai 200135,China)

机构地区：[1]合肥海关技术中心,安徽合肥230022 [2]食品安全分析与检测安徽省重点实验室,安徽合肥230022 [3]上海检验检疫科学技术研究院,上海200135

出　　处：《食品工业科技》2020年第17期243-247,共5页Science and Technology of Food Industry

基　　金：中央引导地方科技发展专项(YDZX20173100004528);安徽省对外科技合作项目(1804b06020349)。

摘　　要：建立了电感耦合等离子体质谱法(ICP-MS)的碰撞模式测定大西洋鲑鱼中Ca、Fe、Zn、Cu、Mn、Pb、As、Cr、Cd、Al10种无机元素含量的方法。采用HNO3-H2O2体系进行微波消解后直接进行测定,以72 Ge、115 In、45 Sc、209 Bi作为内标元素消除基体干扰,选用He碰撞模式的动能歧视(KED)技术消除多原子干扰,对来自挪威、澳大利亚、智利3个产地进口的大西洋鲑鱼中10种无机元素含量进行测定,并结合聚类分析进行归类。结果表明:各元素检出限为0.005~0.058 mg/kg,加标回收率为93.5%~98.2%,相对标准偏差(RSD)为0.73%~3.15%。3个产地大西洋鲑鱼样本间Ca、As含量存在显著差异(P<0.05);挪威与澳大利亚样本之间Ca、Fe、Mn、As、Cr、Al含量差异显著(P<0.05),Cu、Zn、Pb含量无显著差异(P>0.05);挪威与智利样品之间Ca、Cu、As、Cr含量差异显著(P<0.05),Fe、Mn、Zn、Pb、Al含量无显著差异(P>0.05);澳大利亚与智利样品之间Ca、Fe、Cu、Mn、As、Al含量差异显著(P<0.05),Zn、Pb、Cr含量无显著差异(P>0.05)。聚类分析将样品分成三个大类,选择Ca、Fe、As、Cr、Al共5个对区分大西洋鲑鱼产地方差贡献较大的元素含量为自变量建立大西洋鲑鱼3个产地的Fisher判别模型,利用判别模型对样品进行归类,三地样本的判别准确率达到100%。A method to determinate 10 inorganic elements(Ca,Fe,Zn,Cu,Mn,Pb,As,Cr,Cd and Al)in Atlantic salmon by ICP-MS with KED mode was established.In this method,HNO3-H2O2 was used as microwave digestion reagent.72 Ge、115 In、45 Sc、209 Bi were used as internal standard elements to eliminate matrix interference,and kinetic energy discrimination(KED)mode was used to eliminate polyatomic interference with helium as the reaction gas.The contents of 10 inorganic elements in Atlantic salmon from Norway,Australia and Chile were determined,and they were classified by cluster analysis.The results showed that:The detection limit of each element ranged from 0.005 to 0.058 mg/kg with the recovery rate ranged from 93.5%to 98.2%,and the relative standard deviation(RSD)ranged from 0.73% to 3.15%.There was a significant difference in Ca and As contents among three samples of Atlantic salmon(P<0.05).There were significant differences in the contents of Ca,Fe,Mn,As,Cr and Al between Norway and Australia(P<0.05),but no significant differences in the contents of Cu,Zn and Pb(P>0.05).There were significant differences in the contents of Ca,Cu,As and Cr between Norway and Chile(P<0.05),but no significant differences in the contents of Fe,Mn,Zn,Pb and Al(P>0.05).There were significant differences in the contents of Ca,Fe,Cu,Mn,As and Al between Australia and Chile(P<0.05),but no significant differences in the contents of Zn,Pb and Cr(P>0.05).Cluster analysis divided samples into three categories.Five elements,Ca,Fe,As,Cr and Al,which contributed a lot to the variance of the origin of Atlantic salmon,were selected as independent variables to establish Fisher discriminant model of three origins of Atlantic salmon.The discriminant model was used to classify the samples,and the discriminant accuracy of the three regions reached 100%.

关 键 词：大西洋鲑鱼 ICP-MS 无机元素 产地溯源 

分 类 号：TS207.3[轻工技术与工程—食品科学]