脂肪酶催化合成大豆甾醇共轭亚油酸酯的工艺研究  

Lipase-catalyzed synthesis of soybean sterol conjugated linoleate

作　　者：汤桂云 郑玄 陈竞男[1] TANG Guiyun;ZHENG Xuan;CHEN Jingnan(College of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2020年第2期72-77,共6页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家自然科学基金项目“加热油脂体系中大豆甾醇酯的氧化稳定性与氧化机理研究”(31801501);河南省科技攻关项目“大豆甾醇ω-3型脂肪酸酯的合成工艺研究”(182102110024)。

摘　　要：植物甾醇可以有效降低人体血清中的胆固醇含量,但游离型植物甾醇的溶解性较差,为提高其脂溶性,常通过将其与脂肪酸酯化的方法加以改善。采用正相高效液相色谱法对大豆甾醇共轭亚油酸酯进行定性定量分析,以大豆甾醇共轭亚油酸酯产率为考察指标,研究醇酸物质的量比、催化剂用量、反应温度和反应时间对大豆甾醇共轭亚油酸酯产率的影响。通过单因素试验和正交试验,确定最佳工艺条件为:CRL脂肪酶的添加量为6%,醇酸物质的量比为1∶3,酯化反应温度为50℃,酯化反应时间为48 h,在此最佳条件下大豆甾醇共轭亚油酸酯的产率为98.94%。将合成后的产品进行纯化,并采用红外光谱法进行表征。以脂肪酶催化法合成高纯度的大豆甾醇共轭亚油酸酯产品,为新资源食品的开发与高值化利用提供了理论指导和技术支持。Phytosterols can effectively reduce the cholesterol content in human serum.But the solubility of free phytosterols is poor.To improve the liposolubility of phytosterols,esterification with fatty acid is normally adopted.Soybean sterol conjugated linoleate was analyzed quantitatively and qualitatively with normal phase high performance liquid chromatography.Influences of variables,such as the molar ratio of alcohol to acid,catalyst loading,reaction temperature and reaction time,on the yield of soybean sterol conjugated linoleate were investigated by single factor test and orthogonal design.The optimum conditions were as follows:CRL catalyst amount 6%,molar ratio of alcohol to acid 1∶3,reaction temperature 50℃and reaction time 48 h.Under these conditions,the yield of soybean sterol conjugated linoleate was 98.94%.The synthesized product was then purified and characterized by infrared spectroscopy.In this paper,highly purified soybean sterol conjugated linoleate product was synthesized by lipase-catalyzed method,providing a theoretical guidance and technical support for the development and high value utilization of newresource food.

关 键 词：大豆甾醇 共轭亚油酸 脂肪酶 酯化反应 

分 类 号：TS201.2[轻工技术与工程—食品科学]