凝结芽孢杆菌高密度发酵及产孢关键因素研究  

Study on High Cell Density Fermentation and Spore-Forming Conditions of Bacillus Coagulans

作　　者：谭才邓[1] 廖延智[1] 朱美娟[1] 黄亿璇 黄思乐 姚勇芳[1] TAN Caideng;LIAO Yanzhi;ZHU Meijuan;HUANG Yixuan;HUANG Sile;YAO Yongfang(Guangdong Industry Polytechnic,Fapon Biotech Inc.,Dongguan 523808,China)

机构地区：[1]广东轻工职业技术学院,广东广州510300 [2]广东菲鹏生物有限公司,广东东莞523808

出　　处：《食品科技》2020年第7期11-18,共8页Food Science and Technology

基　　金：广东省教育厅项目(2B140703);广东轻工职业技术学院科研项目(HX2018-093)。

摘　　要：以凝结芽孢杆菌TZB-5菌株为研究对象,在高密度发酵及提高产孢率两方面展开探究,旨在为其微生态制剂的制备提供参考。结果表明:麸皮浸出液能大幅度提高菌密度,硫酸铁是促进细胞形成芽孢的关键因素。菌株高密度发酵最佳培养基配方:葡萄糖15 g/L、酵母粉5 g/L、氯化钠5 g/L、麸皮浸出液80%、pH5.5;最佳培养条件:培养温度47℃、转速200 r/min、培养时间20 h,在此条件下发酵的菌密度高达5.5×10~9 cfu/mL;菌株产孢最佳培养基配方:可溶性淀粉10 g/L、豆粕粉10 g/L、酵母粉5 g/L、氯化钠5 g/L、硫酸铁100 mg/L、麸皮浸出液80%、pH5.5,芽孢产量最高达到5.1×10~9 cfu/mL,芽孢率为98.15%。In order to provide reference for microecological preparation,the high cell density fermentation and spore-forming conditions of Bacillus coagulans TZB-5 were studied.The result shows that:the bran extract can greatly increase the cell density,and ferric sulfate is a very important factor for spore forming.The optimal culture medium for high density fermentation is:glucose 15 g/L,yeast extract 5 g/L,sodium chloride 5 g/L,bran extract 80%,pH5.5.The optimal culture conditions is:47℃,200 r/min,shaking for 20 hours.And the cell density is up to 5.5×10~9 cfu/mL.The optimal culture medium for spore formation is:soluble starch 10 g/L,soybean cake powder 10 g/L,yeast extract 5 g/L,sodium chloride 5 g/L,ferric sulfate 100 mg/L,bran extract 80%,pH 5.5,the yield of spores is up to 5.1×10~9 cfu/mL,and the spore-forming rate is 98.15%.

关 键 词：凝结芽孢杆菌 高密度发酵 芽孢 微生态制剂 

分 类 号：TS201.3[轻工技术与工程—食品科学]