HS-SPME-GC-MS分析小黄姜精油及油树脂挥发性香气成分  

Analysis of Volatile Aroma Constituents of Ginger Essential Oils and Oleoresin by HS-SPME-GC-MS

作　　者：张璐璐 吴建文[2] 黎贵卿[2] 刘慧勤 陆顺忠[2] 李秋庭[1] ZHANG Lu-lu;WU Jian-wen;LI Gui-qing;LIU Hui-qin;LU Shun-zhong;LI Qiu-ting(Light Industry and Food Engineering College,Guangxi University,Nanning 530004,Guangxi,China;Guangxi Zhuang Autonomous Region Forestry Research Institute,Nanning 530002,Guangxi,China)

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530004 [2]广西壮族自治区林业科学研究院,广西南宁530002

出　　处：《食品研究与开发》2020年第19期166-172,共7页Food Research and Development

摘　　要：以西林小黄姜为原料,通过水蒸气蒸馏得精油(ginger essential oils,GEO)和石油醚、无水乙醇分别索氏提取得石油醚油树脂(oleoresin extracted by petroleum ether,PEEO)、无水乙醇油树脂(oleoresin extracted by absolute ethanol,AEEO)共3种姜油,采用顶空-固相微萃取(headspace solid phase micro-extraction,HS-SPME)结合气相色谱质谱(gas chromatography-mass spectrometer,GC-MS)对其挥发性香气成分进行分析,探讨萃取头、萃取时间和解析时间对萃取效果的影响,结果表明:采用100μm PDMS萃取头,精油萃取3.5 min,解析3 min,油树脂萃取7.5 min,解析2 min,效果最佳。在最佳条件下GEO、PEEO、AEEO分别鉴定出44、41、44种香气成分,组成和含量均具有一定差异,呈香各具特色。Using Xilin ginger as raw material,ginger essential oils(GEO)were extracted by hydrodistillation,petroleum ether extracted by petroleum ether(PEEO)and oleoresin extracted by absolute ethanol(AEEO)both by soxhlet methods.The volatile aroma components were analyzed by headspace solid phase micro-extraction(HS-SPME)combined with gas chromatography-mass spectrometer(GC-MS),and the effects of extraction head,extraction time and desorption time on extraction efficiency were discussed.The results showed that:the best results were extraction for 3.5 min and desorption for 3 min of essential oil,extraction for 7.5 min and desorption 2 min of oleoresin by using 100μm PDMS extraction head.Under the optimal conditions,GEO,PEEO and AEEO identified 44,41 and 44 aroma components,respectively,and the differences in composition and content made them have their own aroma characteristics.

关 键 词：西林小黄姜 姜精油 姜油树脂 挥发性香气成分 顶空固相微萃取 

分 类 号：O657.63[理学—分析化学] TQ654.2[理学—化学]