Daifan San intervenes in forkhead box P3 and the interleukin(IL)-23/IL-17A signaling pathway to help prevent and treat primary biliary cirrhosis  

作　　者：Zhan Kai Xu Yan Han Mengling Cheng Liangbin 

机构地区：[1]The Second Clinical College of Guangzhou University of Chinese Medicine,Guangzhou 510000,China [2]Department of Internal Medicine,Hubei Rongjun Hospital,Wuhan 430079,China [3]Department of Hepatology,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510000,China [4]Department of Hepatology,Hubei Provincial Hospital of Chinese Medicine,Wuhan 430061,China

出　　处：《Journal of Traditional Chinese Medicine》2020年第4期571-583,共13页中医杂志（英文版）

基　　金：The Science and Technology Bureau of Wuhan,China(No.2015061701011646);the Key Projects of the Hubei Provincial Education Department(No.D20112001)。

摘　　要：OBJECTIVE:To investigate the mechanism by which Daifan San(DFS)prevents and treats primary biliary cirrhosis(PBC)via the forkhead box P3(FoxP3)and interleukin(IL)-23/IL-17A signaling pathways.METHODS:Ninety C57BL/6 mice were randomly divided into the control,model,DFS low-dose,DFS middle-dose,DFS high-dose and ursodeoxycholic acid(UDCA)groups(n=15 per group).A mouse model of PBC was induced using polyinosinic polycytidylic acids(poly I:C).Lymphocyte subset expression in the peripheral blood was analyzed via flow cytometry.The inflammatory cytokines and antimitochondrial autoantibody(AMA)levels were detected via enzyme-linked immunosorbent assays.The expressions and location of typeⅠcollagen,typeⅢcollagen,cytokeratin 19 and FoxP3 in the liver tissue were evaluated via immunohistochemistry.FoxP3,IL-23 and IL-17 expressions in the peripheral blood and liver tissue were evaluated via real-time polymerase chain reaction and western blotting.RESULTS:IL-17,IL-23,IL-8,IL-33,TNF-α,and AMA expressions were significantly increased in the model group and decreased in the DFS and UDCA groups.Conversely,Treg cell and FoxP3 expressions were significantly decreased in the model group and increased in the DFS and UDCA groups.The IL-23/IL-17A signaling pathway was closely correlated with chronic inflammation of the bile duct in PBC and functional deletion of Treg cells,leading to reduced FoxP3 levels and mediating the loss of tolerance in PBC.CONCLUSION:DFS may delay the occurrence and relieve the symptoms of PBC by downregulating IL-23/IL-17A signaling pathway expression and upregulating FoxP3 expression.

关 键 词：Liver cirrhosis biliary INTERLEUKIN-23 INTERLEUKIN-17 Signal transduction FoxP3 protein mouse Daifan San 

分 类 号：R575.22[医药卫生—消化系统]